In this study,we characterized a set of 46 accessions of soybean from Russian Far East and 44 ac-cessions from Heilongjiang Province using 40 SSR primers,to evaluate their genetic diversity and population struc-ture.The results showed that,the genetic diversity of soybeans from Russian Far East was higher than that of Hei-longjiang Province.The germplasm resources were divided into 3 clusters by NJ phenogram depicting and Structure software.Each cluster contained accessions from Russia and Heilongjiang,but the majority were likely originated from the same region.The majority of cluster Ⅰ,cluster Ⅱ were the accessions from Russia,and cluster Ⅲ were from Heilongjiang.In the present study,these germplasm resources were not divided in accordance with their geo-graphical origin.It indicated that the relationship between soybean germplasm from Russia and Heilongjiang was close.It was necessary to introduce a wider range of germplasm from Russian Far East to broaden the genetic basis of soybean in Heilongjiang Province.