Comparative transcriptome analysis of differences in seed germination characteristics between two soy-bean varieties
Soybean seed germination determines the seedling establishment and developmental process.Rapid germination and high germination rate are essential for achieving high yield in soybean production.Based on observ-ing seed coat micromorphology and detecting the main physiological indexes at the early stage of germination,it was aimed to screen differentially expressed genes by transcriptome analysis with Heinong 35 and its mutant line M at the exposure stage(24 h after imbibition)so as to further clarify the mechanism of soybean seed germination.Re-sults showed that there were significant differences in water absorption and germination rate.M had longer hilum and thinner seed coat than Heinong 35 by scanning electron microscopy,which might be the main reason that M ab-sorbed water fast in the early germination stage.During germination,the abscisic acid(ABA)content gradually de-creased,while the soluble protein content first decreased and then increased,and the soluble sugar content present-ed the trend of increase-decrease-increase with"S"shape.By transcriptome analysis,1122 differentially expressed genes were screened,of which 478 were up-regulated and 644 were down-regulated.The differentially expressed genes were mainly enriched in the photosynthetic pathway.22 candidate genes related to the photosynthetic path-way were screened and down-regulated in M.It was deduced that these down-regulated genes in M might cause slower photomorphogenesis and then lead to lower germination rates.These results should be helpful for elucidating the regulatory mechanism of soybean seed germination.
万方数据
维普
