A rapid technology for the propagation of soybean regenerated shoot using grafting with cotyledon-only plant as stock
There are some root-related problems including difficult rooting and inadequate rooting of regener-ated shoots,microbial contamination of regenerated roots,low survival rate and poor growth of transplanted plants in the soybean tissue culture process,which significantly limit the efficiency improvement of genetic transformation.In this study,we established a simple and efficient propagation method for soybean regenerated shoot using a grafting system with cotyledon-only plant(COP)as stock.The results showed that the survival rate of the regenerated shoot in the grafting union with 4-6 d old COP as stock and the regenerated shoot with no root,few roots or microbially contaminated root as scion was as high as 79.8%±4.9%.After cutting a regenerated shoot into 2-3 stem segments and then grafting them with COP stock,the grafted shoots totally produced 1.8-fold more seeds(234.5±39.1)than that of an individual plant(83.7±13.2)derived from rooting induction in the tissue culture system.In conclusion,the grafting method using COP as stock and regenerated shoot as scion omits the root induction of the regenerated shoot in the tissue culture system;and resolves the problems of no rooting,few rooting and microbial contamination of roots in soybean tissue culture,improves the survival rate,and increases the seed number of the regenerated plants.Hence,the propagation efficiency was obviously elevated.This technology facilitates the efficient genetic transformation and engineered propagation in soybean and provides new insight into other dicots with problems in the rooting process of regenerated shoot in the tissue culture.
万方数据
维普
