Investigation on the relationship between stem and leaf metabolites and tuber morphology of Cyperus esculentus
To better utilize the stem and leaf of Cyperus esculentus and facilitate its crop breeding,relationship was studied between phenotypic features and stem-and-leaf metabolites,by investigating phenotypic parameters of 10 cultivars.The parameters including leaf width,tuber width,tuber thickness and tuber height,were compared and analyzed.Stem and leaf metabolites were qualitatively and quantitatively analyzed by ultra-high performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS).Results showed that 10 cultivars of C.esculentus were divided into 2 phenotypes,namely wide-leaf round-tuber type and narrow-leaf long-tuber type.Their leaf width was positively correlated with tuber width and tuber thickness.Relative contents of 28 main metabolites were discovered and investigated including flavane-3-ols,caffeic acids and flavonoids.Results showed that the contents of 4 flavonoids(luteolin 7-O-diglucuronide,luteolin 8-C-glucoside,luteolin 7-glucuronide,and luteolin)were sig-nificantly higher in wide-leaf round-tuber C.esculentus leaves.Biosynthesis pathways of the 4 differential flavo-noids metabolites were deduced and mapped using KEGG and PlantCyc.It was predicted that the wide-leaf round-tuber type was more drought-tolerant than the narrow-leaf long-tuber ones.
万方数据
维普
