Effects of nitrogen application rates on enzyme activities related to carbon and nitrogen metabolism,yield and quality of peanut in chernozem
To determine the optimal nitrogen application rate for chernozak peanuts,Jihua 25 was selected as the experimental material and subjected to six different levels of nitrogen fertilizer.These levels included a control group with no added nitrogen(0 kg/hm2,CK),traditional fertilization at 112.5 kg/hm2(N1),a 20%reduction in nitro-gen at 90 kg/hm2(N2),a 30%reduction in nitrogen at 79.5 kg/hm2(N3),a 40%increase in nitrogen at 159 kg/hm2(N4)and double the amount of applied nitrogen at 225 kg/hm2(N5).The impact of varying application rates on en-zyme activity,yield,and quality related to carbon and nitrogen metabolism were assessed.The results indicated that the use of optimal nitrogen fertilizer resulted in an increase in the activities of nitrate reductase(NR),glutamine syn-thetase(GS),glutamate dehydrogenase(GDH),sucrose synthetase(SS)and sucrose phosphate synthetase(SPS).Con-versely,excessive application of nitrogen fertilizer resulted in a decrease in the activities of NR,GS,GDH,SS and SPS.The yield of each nitrogen application treatment exceeded that of the no-fertilizer control.Among all treat-ments,the peanut yield was highest when traditional fertilizer was applied with a 20%reduction in nitrogen(N2),re-sulting in a 9.21%increase compared to the control and a 1.57%increase compared to traditional fertilizer alone.These results indicated that N2 treatment could not only meet the nitrogen demand of peanut growth and develop-ment,but also ensure the activity of enzymes related to carbon and nitrogen metabolism in leaves,enhance the abili-ty of peanut to absorb and utilize nitrogen,and increase the number of double-kernel pods per plant,100-pod weight and 100-kernel weight per plant,reduce the number of immatured pods and single-kernel pods per plant,in-creasing the number of double-kernel per plant,100-pod weight and 100-kernel weight,the peanut yield was final-ly increased.
peanutsnitrogen levelyieldqualitycarbon and nitrogen metabolizing enzymes
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维普
