首页|大豆抗大豆花叶病毒SC7和SC15株系基因的全基因组关联分析

大豆抗大豆花叶病毒SC7和SC15株系基因的全基因组关联分析

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大豆花叶病毒(soybean mosaic virus,SMV)引起的病害是危害大豆生产和品质的主要病害之一,解析大豆抗病遗传机制能为利用大豆抗病品种防治该病害奠定基础.本研究选用193份种质资源,分别接种大豆花叶病毒株系SC7和SC15后进行抗感表型鉴定,结合656977个SNP标记进行全基因组关联定位,结果表明与SC7抗性显著相关位点有一个,位于15号染色体;与SC15抗性显著相关位点有10个,分别位于2、3、4、5、9、12、14、17和19号染色体.这些位点均为新的抗病位点.此外,本研究参考Williams82.a2基因组找出每个抗病位点上下游各50 kb内的基因,利用基因组核苷酸多态性信息,将有SNP落点的基因作为最可能参与抗SMV的候选基因并进行qRT-PCR,分析发现,基因Glyma.02g163300、Glyma.19g027200和Glyma.19g057700可能参与了大豆对SC15的抗性.本研究结果将为大豆抗病种质培育提供重要的基因资源.
Genome-wide association mapping of resistance to soybean mosaic virus SC7 and SC15 in soybean
Soybean mosaic virus(SMV)disease is one of the main diseases affecting production and quality of soybean.Analyzing the genetic basis of soybean disease resistance can lay a foundation for controlling this disease by using soybean resistant varieties.In this study,193 germplasm resources were performed phenotypic identifica-tion by inoculating soybean mosaic virus strains SC7 and SC15,these phenotypic data and 656977 SNP markers of germplasms were used to perform genome-wide association mapping.One significant SNP-trait association with SC7 resistance was detected on chromosome 15.Ten significant SNP-trait association with SC15 resistance were de-tected and located on chromosomes 2,3,4,5,9,12,14,17 and 19,respectively.These resistance SNP loci maybe new loci.In addition,all genes were found in the upstream and downstream 50 kb of each resistance locus by refer-ring to the Williams82.a2 genome,some genes with SNP difference were selected as the candidate genes for resis-tance and analyzed by qRT-PCR.The results showed that genes Glyma.02g163300,Glyma.19g027200,and Gly-ma.19g057700 might be involved in resistance to SC15 in soybean.The results of this study was expected to pro-vide important genetic resources for soybean germplasm cultivation.

soybeansoybean mosaic virusresistance geneGWAScandidate genes

牛景萍、杨彩妮、赵晋忠、陈钰涛、王迎新、李丽、王敏、岳爱琴、杜维俊

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山西农业大学生命科学学院,山西 晋中,030801

山西农业大学农学院,山西 晋中,030801

山西农业大学基础部,山西 晋中,030801

大豆 大豆花叶病毒病 抗病基因 关联分析 候选基因

山西种业创新良种联合攻关项目国家重点研发计划山西农业大学科技创新基金项目山西省优秀博士来晋工作奖励资金科研项目山西农业大学省部共建有机旱作农业国家重点实验室自主研发项目山西农业大学育种工程项目山西农业大学农学院育种工程重点培育专项

2022xczx032021YFD1600601-22018YJ29SXYBKY2018025202105D121008-3-8YZGC096YZ2021-05

2024

10.19802/j.issn.1007-9084.2022244

中国油料作物学报
中国农业科学院油料作物研究所

中国油料作物学报

CSTPCD北大核心
影响因子：1.296
ISSN：1007-9084
年,卷(期)：2024.46(1)
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