Soybean mosaic virus(SMV)disease is one of the main diseases affecting production and quality of soybean.Analyzing the genetic basis of soybean disease resistance can lay a foundation for controlling this disease by using soybean resistant varieties.In this study,193 germplasm resources were performed phenotypic identifica-tion by inoculating soybean mosaic virus strains SC7 and SC15,these phenotypic data and 656977 SNP markers of germplasms were used to perform genome-wide association mapping.One significant SNP-trait association with SC7 resistance was detected on chromosome 15.Ten significant SNP-trait association with SC15 resistance were de-tected and located on chromosomes 2,3,4,5,9,12,14,17 and 19,respectively.These resistance SNP loci maybe new loci.In addition,all genes were found in the upstream and downstream 50 kb of each resistance locus by refer-ring to the Williams82.a2 genome,some genes with SNP difference were selected as the candidate genes for resis-tance and analyzed by qRT-PCR.The results showed that genes Glyma.02g163300,Glyma.19g027200,and Gly-ma.19g057700 might be involved in resistance to SC15 in soybean.The results of this study was expected to pro-vide important genetic resources for soybean germplasm cultivation.