首页|辽宁省花生焦斑病病原菌分离鉴定及其生物学特性

辽宁省花生焦斑病病原菌分离鉴定及其生物学特性

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为明确辽宁省花生焦斑病的病原菌,采用组织分离法分离病原菌,依据科赫氏法则进行致病力测定,并对菌落形态以及子囊壳、子囊和子囊孢子的形态进行观察,对病菌的LSU、ITS和Tub2基因进行测序,然后做多基因联合建树分析.结果发现,花生焦斑病菌株菌落扁平,气生菌丝稀疏,菌落边缘呈叶状,在生长初期菌丝体最初呈白色,然后从菌落中心开始向外逐渐变成黑褐色;子囊孢子呈椭圆形,具有凝胶状鞘,有隔;结合多基因序列联合的系统发育分析,确定引起辽宁省花生焦斑病的病原菌为Leptosphaerulina arachidicola.病菌的生物学特性测定结果显示,花生焦斑病病原菌的菌丝最适生长温度为25℃、最适生长pH为5,最适生长碳源为可溶性淀粉,最适生长氮源为胰蛋白胨,致死温度为50℃.
Pathogen isolation,identification and biological characteristics of peanut scorch spot in Liaoning Province
In order to define the pathogen of peanut scorch spot disease in Liaoning Province,tissue isolation method was utilized.Its pathogenicity was determined according to Koch's postulates.Morphology of colony,peri-thecia,asci and ascospores was observed.LSU,ITS and Tub2 genes of pathogen were sequenced for multi-gene joint construction analysis.Results showed that the pathogen formed flat colonies with sparse aerial mycelium and smooth and lobate margins.Mycelium was initially white and then changed to black brown from the center of the col-ony.Ascospores were ellipsoid to oblong with a gelatinous sheath and septum.Based on the combined phylogenetic analysis of LSU,ITS and Tub2 gene sequences,the pathogen was identified to be Leptosphaerulina arachidicola causing peanut scorch spot in Liaoning Province.Biological characteristics showed that a suitable temperature for pathogen mycelial growth was 25℃,at the optimal pH 5,under the optimal carbon source of soluble starch,and the optimal nitrogen source of pancreatic peptone.Its lethal temperature of the mycelial was 50℃.

scorch spot of peanutLeptosphaerulina arachidicolamorphology identificationmolecular iden-tificationbiological characteristics

韩宁、孙继军、史普想、孙泓希、王一波、王海新

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辽宁省沙地治理与利用研究所,辽宁 阜新,123000

花生焦斑病 Leptosphaerulina arachidicola 形态学鉴定 分子鉴定 生物学特性

辽宁省农科院花生育种与栽培学科建设项目辽宁小粒花生产业发展关键技术协同创新国家重点研发计划项目

2022DD1961322022XTCX05022020YFD1000905

2024

10.19802/j.issn.1007-9084.2022211

中国油料作物学报
中国农业科学院油料作物研究所

中国油料作物学报

CSTPCD北大核心
影响因子：1.296
ISSN：1007-9084
年,卷(期)：2024.46(1)
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