中国油料作物学报2024,Vol.46Issue(1) :191-200.DOI:10.19802/j.issn.1007-9084.2022269

内生菌YYC155诱导油茶抗病性及转录组分析

Disease resistance and transcriptome analysis of Camellia oleifera induced by endophyte YYC155

周嫒婷 王芳 彭睿琦 伍建榕 马焕成
中国油料作物学报2024,Vol.46Issue(1) :191-200.DOI:10.19802/j.issn.1007-9084.2022269

内生菌YYC155诱导油茶抗病性及转录组分析

Disease resistance and transcriptome analysis of Camellia oleifera induced by endophyte YYC155

周嫒婷 1王芳 1彭睿琦 1伍建榕 2马焕成1
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作者信息

  • 1. 西南林业大学林学院,云南 昆明, 650224
  • 2. 西南林业大学林学院,云南 昆明, 650224;西南林业大学生物多样性保护学院,云南 昆明, 650224
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摘要

为探究油茶响应内生菌YYC155诱导抗病性的机制,以2年生油茶苗为试验材料,测定YYC155诱导油茶的生防效果和抗病性防御酶活性的变化,并利用RNA-Seq技术对诱导15 d的样品进行转录组测序和分析.结果表明,经YYC155诱导后油茶对炭疽病的防效显著,为48.28%,且防御性酶活性被激活.同时,CK-vs-YYC155样本共筛选到差异表达基因(DEGs)1655个,DEGs在GO功能中被涉及生物过程、分子功能和细胞组分,被富集到102个KEGG通路上,其中呈显著富集的是:MAPK植物信号通路,植物激素信号转导,光合作用,植物-病原互作;另外,与抗病相关基因AUX1、GID1、PP2C、JAR1等大量上调表达.以上结果证实了YYC155能较好的诱导油茶抗病防御性系统,是1株具有潜在应用价值的生防内生菌.

Abstract

To explore the mechanism of Camellia oleifera response to endophytic YYC155 inducing disease re-sistance,2-year-old C.oleifera was used as material to determine the biological control effect of YYC155 on an-thracnose of C.oleifera and the changes of disease resistance defense enzyme activity after induction,and RNA Seq technology was used to sequence and analyze the transcriptome of samples induced for 15 days.The results showed that YYC155 had a significant control effect on anthracnose of C.oleifera,which was 48.28%,and the defensive en-zyme activity was activated.Moreover,1655 differentially expressed genes(DEGs)were screened from CK vs YYC155 samples.DEGs were involved in biological processes,molecular functions and cell components in GO functions,and were enriched in 102 KEGG pathways,among which MAPK plant signal pathway,plant hormone sig-nal transduction,photosynthesis,and plant pathogen interaction were significantly enriched;In addition,resistance related genes AUX1,GID1,PP2C,JAR1,etc.were up-regulated.The above confirmed that YYC155 can better in-duce the disease resistance and defense system of C.oleifera,and it is a biocontrol endophytic strain with potential application value.

关键词

油茶/内生菌/诱导/抗病性/转录组

Key words

Camellia oleifera/endophytic bacteria/induced/disease resistance/transcriptome

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基金项目

国家重点研发计划(2019YFD100200X)

国家自然科学基金项目(31860208)

国家自然科学基金项目(31560207)

出版年

2024
中国油料作物学报
中国农业科学院油料作物研究所

中国油料作物学报

CSTPCDCSCD北大核心
影响因子:1.296
ISSN:1007-9084
参考文献量46
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