Soil sealing treatment is an effective chemical weed control method in rapeseed field.To evaluate the effects of different soil-applied herbicides on soil microorganisms in rapeseed field,16S rRNA amplicon se-quencing was used to analyze the changes in soil microbial structure and diversity after treatment with four common-ly used soil-applied herbicides alachlor,acetochlor,metochlor and s-metolachlor.The results showed that soil-ap-plied herbicide treatments could significantly reduce soil bacterial diversity in rapeseed fields,and alachlor had the greatest effect on soil bacterial diversity.However,after a period of herbicide treatment,the diversity and richness of the soil bacterial community gradually recovered.Soil-applied herbicide treatment had great influence on Actino-mycetes,Acidobacteria and others in rapeseed soil,and alachlor treatment showed the greatest change in soil bacte-ria at phylum level compared with other herbicides.After 60 days of treatment with soil-applied herbicides,the abundances of functional bacteria related to pollutant degradation,denitrification,photosynthesis,pathogen growth inhibition and rhizosphere growth promotion were increased,whereas functional bacteria associated with the absorp-tion of nutrients and excretion of metabolites were reduced.Overall,the results indicated that the soil-applied herbi-cides had a certain effect on soil microbial diversity and community structure in rapeseed field,but the effect of her-bicides on soil microbial diversity gradually decreased along with the time of treatment.
国家科技期刊平台
NSTL
万方数据
