印度梨形孢-花生共生体系建立及对花生的促生作用
Establishment of Piriformospora indica-peanut symbiosis system and its promoting effect on peanut growth
高洁 1田培聪 1党悦嘉 2李昕芮 3王趁 2刘闯 2黄玉茜2
作者信息
- 1. 沈阳农业大学土地与环境学院,辽宁 沈阳,110866
- 2. 沈阳农业大学植物保护学院,辽宁 沈阳,110866
- 3. 沈阳农业大学植物保护学院,辽宁 沈阳,110866;吉林大学植物科学学院,吉林 长春,130062
- 折叠
摘要
印度梨形孢(Piriformospora indica)是一种内生真菌.为提高花生产量,探究印度梨形孢对花生的促生作用,以花生(粤油7号)为材料,采用室外盆栽实验,建立印度梨形孢与花生共生体系,调查对花生植株的农艺性状、产量、光合特性、丙二醛含量、根系活力和抗氧化酶活性等生理指标的影响.结果发现:采用菌丝浇灌的方式接种印度梨形孢,成功建立了与花生的共生体系,侵染率为63%.苗期时,叶片叶绿素SPAD值和净光合速率显著增加24.17%和19.66%,蒸腾速率比未接种印度梨形孢的花生植株22%;在苗期与成熟期,接种印度梨形孢的花生主根长、地下部鲜重、地下部干重、根系活力和叶片的过氧化氢酶(CAT)、过氧化物酶(POD)活性显著提高,丙二醛含量分别显著降低24.27%和22.12%;接种印度梨形孢提高了花生产量.
Abstract
To improve peanut yield,growth-promoting effect of endophytic fungus Piriformospora indica on peanut plants was investigated in pots.Peanut cv.Yueyou 7 was used as experimental material.Symbiosis system between P.indica and peanut was established.Agronomic traits,yield,photosynthetic characteristics,malondialde-hyde content,root activity and antioxidant enzyme activity of peanut were studied.Results showed that the symbio-sis system of P.indica and peanut was established successfully by inoculating P.indica with hyphae.The infection rate was 63%.At seedling stage,net photosynthetic rate and chlorophyll SPAD value of peanut leaves were signifi-cantly increased by 24.17%and 19.66%,and transpiration rate was 22%lower than that of uninoculated peanut plants.At seedling and mature stages,compared with uninoculated P.indica,root length,underground fresh weight,underground dry weight,root activity and leaf catalase peroxidase activity were significantly increased after inocula-tion.Content of malondialdehyde was significantly reduced by 24.27%and 22.12%at seedling and mature stages.Peanut yield was increased by inoculation with P.indica.
关键词
花生/印度梨形孢/农艺性状/抗氧化酶/产量Key words
peanut/Piriformospora indica/agronomic characteristics/antioxidant enzymes/yield引用本文复制引用
基金项目
国家重点研发计划(2018YFD0201007-2)
辽宁省普通高校基本科研面上项目(LJKMZ20221044)
辽宁省北镇市花生科技特派团项目(2022JH5/10400053)
出版年
2024
国家科技期刊平台
NSTL
万方数据