Identification method of ferritin absorption/activation ability of peanut(Arachis hypogaea L.)varieties under hydroponic condition
In order to establish a rapid method to identify the absorption and activation ability of iron in differ-ent peanuts,the iron deficiency cultivars Nongda 818,Luhua 11,Shanhua 11,and the iron sensitive cultivars Luhua 12,ICG6848,and Baisha 1016 were employd in iron deficiency nutrient solution instead of soil.The yellow-ing time of new leaves in iron deficiency environment was recorded.After the new leaves of all peanut seedlings turned yellow,the iron supply was resumed.The seedlings were treated with 20 µmol/L FeSO4 for 2 d and 4 d,25 µmol/L FeSO4 for 2 d and 3 d,and 30 µmol/L FeSO4 for 1 d,2 d and 4 d,the increase of chlorophyll value(ΔSPAD),dry weight and iron uptake were measured to identify the iron uptake capacity.The iron activation abili-ty of peanut was evaluated at 25 µmol/L Fe2(SO4)3 for 2 d and 4 d,30 µmol/L Fe2(SO4)3 for 2 and 3 d,and 35 µmol/L Fe2(SO4)3 for 1 day,2 d and 4 d.Results showed that the seedlings of all peanuts showed yellowing on the 10th day of iron deficiency treatment.Then,25 µmol/L FeSO4 was used to iron supply for 2 days,which led to in-crease of SPAD value,while iron uptake per plant were significantly different between iron deficiency tolerant and iron sensitive cultivars.When treated with 30 µmol/L Fe2(SO4)3 for 2 days,the increase of SPAD value and iron ac-tivation per plant were also significantly different between the deficient tolerant and sensitive cultivars.Therefore,considerting peanut iron absorption/activation ability,the increase of seedling SPAD,iron uptake per plant and/or activation per plant could be used as indicators after iron supply(25 µmol/L FeSO4 or 30 µmol/L Fe2(SO4)3 for 2 days)when seedlings have been etiolated.
国家科技期刊平台
NSTL
万方数据
