摘要
为建立快速鉴定不同花生品种铁素吸收、活化能力的方法,以耐缺铁花生品种农大818、鲁花11、山花11号,铁敏感花生品种鲁花12、ICG6848、白沙1016为材料进行水培试验.以缺铁营养液培养花生幼苗,观察并记录在缺铁环境中幼苗新叶黄化时间;待幼苗新叶全部黄化后恢复供铁,分别以20 µmol/L FeSO4处理2 d/4 d,25 µmol/L FeSO4处理2 d/3 d,30 µmol/L FeSO4处理1 d/2 d/4 d,通过测定叶绿素值增加量(ΔSPAD)、干物重、铁吸收量等指标以鉴定花生铁素吸收能力;以25 µmol/L Fe2(SO4)3处理2 d/4 d,30 µmol/L Fe2(SO4)3处理2 d/3 d,35 µmol/L Fe2(SO4)3处理1 d/2 d/4 d以鉴定花生铁素活化能力.结果表明,所有花生品种的幼苗在缺铁处理第10 d均出现了黄化现象,此时用25 µmol/L FeSO4恢复供铁处理2 d后,耐缺铁品种和铁敏感品种间ΔSPAD、单株铁吸收量差异显著;用30 µmol/L Fe2(SO4)3处理2 d后,耐缺铁品种和铁敏感品种间ΔSPAD、单株铁活化量也有显著差异.因此可在缺铁黄化后补充25 µmol/L FeSO4或30 µmol/L Fe2(SO4)3,用处理2 d时花生幼苗ΔSPAD、单株铁吸收量或单株活化量作为评价花生铁素吸收/活化能力的指标.
Abstract
In order to establish a rapid method to identify the absorption and activation ability of iron in differ-ent peanuts,the iron deficiency cultivars Nongda 818,Luhua 11,Shanhua 11,and the iron sensitive cultivars Luhua 12,ICG6848,and Baisha 1016 were employd in iron deficiency nutrient solution instead of soil.The yellow-ing time of new leaves in iron deficiency environment was recorded.After the new leaves of all peanut seedlings turned yellow,the iron supply was resumed.The seedlings were treated with 20 µmol/L FeSO4 for 2 d and 4 d,25 µmol/L FeSO4 for 2 d and 3 d,and 30 µmol/L FeSO4 for 1 d,2 d and 4 d,the increase of chlorophyll value(ΔSPAD),dry weight and iron uptake were measured to identify the iron uptake capacity.The iron activation abili-ty of peanut was evaluated at 25 µmol/L Fe2(SO4)3 for 2 d and 4 d,30 µmol/L Fe2(SO4)3 for 2 and 3 d,and 35 µmol/L Fe2(SO4)3 for 1 day,2 d and 4 d.Results showed that the seedlings of all peanuts showed yellowing on the 10th day of iron deficiency treatment.Then,25 µmol/L FeSO4 was used to iron supply for 2 days,which led to in-crease of SPAD value,while iron uptake per plant were significantly different between iron deficiency tolerant and iron sensitive cultivars.When treated with 30 µmol/L Fe2(SO4)3 for 2 days,the increase of SPAD value and iron ac-tivation per plant were also significantly different between the deficient tolerant and sensitive cultivars.Therefore,considerting peanut iron absorption/activation ability,the increase of seedling SPAD,iron uptake per plant and/or activation per plant could be used as indicators after iron supply(25 µmol/L FeSO4 or 30 µmol/L Fe2(SO4)3 for 2 days)when seedlings have been etiolated.
基金项目
国家重点研发计划(SQ2018YFD100007)
山东省农业良种工程项目(2020LZGC001)
国家科技期刊平台
NSTL
万方数据