棉花-花生轮作下的根系土壤细菌群落结构及功能分析
Bacterial community structure and function in root soil under cotton-peanut rotation
崔福洋 1侯献飞 2苗昊翠 2贾东海 2顾元国 2陈晓露 1李强 2施俊杰 2汪天玲 2高君 2胡凤仪 2宋贤明 2张云 2罗玉涛 2黄奕 2尚随腾 2张帆2
作者信息
- 1. 伊犁师范大学,新疆 伊犁,835000
- 2. 新疆农业科学院经济作物研究所,新疆 乌鲁木齐,830091
- 折叠
摘要
为了提升棉花和花生产量,开展棉花-花生轮作模式研究,分析轮作模式下土壤根系微生物的群落结构与功能.以棉花连作、花生连作、棉花花生轮作、花生棉花轮作和休耕为研究对象,探明轮作后作物根际土壤微生物群落结构变化,利用16S rRNA基因高通量测序技术分析根系细菌群落结构与功能.结果发现:轮作提高了根系细菌群落的多样性;土壤样本中共获得5 009 952个有效序列,休耕模式下OTU(operational taxonomic units)含量最多.在轮作种植模式中变形菌门、酸杆菌门和放线菌门为优势菌门;其次轮作模式增加了厚壁菌门、拟杆菌门和芽单胞菌门的丰富度.根据功能不同,将菌群共划分为8类,轮作出现较多微生物基因遗传相关的功能类群,整体以代谢相关的类群居多,与人类和植物病原菌的功能蛋白组富集明显.由此得出,棉花,花生轮作能够提高微生物丰富度,增加多样性,改变土壤微生物群落结构,促进作物根系对营养物质的吸收,提高作物产量.
Abstract
To enhance the yields of cotton and peanut,bacterial community structure and function in root soil under cotton-peanut rotation were studied.Five treatments were investigated,as continuous cotton cultivation,con-tinuous peanut cultivation,cotton-peanut rotation,peanut-cotton rotation,and fallow land.High-throughput gene sequencing of 16S rRNA was used to analyze bacterial community structure and function.Results revealed that crop rotation increased the diversity of root-associated bacterial communities.A total of 5 009 952 valid sequences were obtained from soil samples,with the highest number of OTUs(operational taxonomic units)under fallow land treat-ment.In rotational cropping systems,Proteobacteria,Acidobacteria,and Actinobacteria were identified as dominant phyla.Additionally,richness of Firmicutes,Bacteroidetes,and Gemmatimonadetes were increased under rotational patterns.Based on different functions,the microbial groups were categorized into 8 classes.Under rotations modes,higher prevalence of functional groups related to microbial genetic inheritance was found.Metabolic-related groups were predominant overall,and significant enrichment of functional protein sets were found associated with human and plant pathogens.These results indicated that cotton-peanut rotation could increase microbial richness and di-versity,alter soil microbial community structures,promote nutrient absorption by crop roots,and ultimately enhance crop yields.
关键词
棉花-花生/轮作/细菌群落/PICRUSt功能预测Key words
cotton-peanut/crop rotation/bacterial community/PICRUSt function prediction引用本文复制引用
基金项目
国家重点研发计划(2022YFD1000105)
自治区"天山英才"培养计划现代农牧业发展高层次人才项目(2023SNGGGCC018)
国家花生产业技术体系建设项目(CARS-13)
新疆维吾尔自治区油料产业技术体系建设项目(XJARS-05)
出版年
2024
国家科技期刊平台
NSTL
万方数据