New germplasm of dwarf colored rape created by microspore culture in Brassica napus DW871
Colored rape has oil and ornamental value,which has attracted more and more attention due to its easy planting,bright petal color and long flowering time,etc.However,it has been reported that colored rape is mainly high in erucic acid and glucosinolate,which is not suitable for ornamental and oil application.Therefore,it is one of the breeding goals of ornamental rape to breed new colored rape germplasm with good quality and suitable plant type.In this study,DW871 that has special inflorescence characteristics and great ornamental value was used as the base material.After crossing with common colored high stalk rape,F1 generation was used to create new col-ored rape which has DW871 specific inflorescence traits and excellent quality.The results showed that the embryo yield rate was up to 312-507 embryos/bud by using microspore culture technology in the early stage,and finally a total of 14 colored rape germplasms were obtained,including 5 plants of white color flower,4 plants of orange color flower and 5 plants of kumquat color flower.Through character investigation and quality analysis,we selected all DH plants and found that Y57 is an excellent new germplasm that has dwarf stalk,kumquat color petal and"double low"quality,being familiar with special inflorescence of DW871.The plant height of Y57 is 88 cm,which has 18 primary branches,39%of oil content,25.96%of protein content,0.95%of erucic acid and 28.15 μmol/g·pie of glu-cosinolate.Y57 is a new colored germplasm of DW871 that may have ornamental and oil value and high application prospect.