Preliminary analysis of hormone sensitivity and dwarf mutation mechanism of dwarf mutant DW871 in Brassica napus L.
In this study,the dwarf mutant DW871 and high-stem rapeseed homologous HW871 were used to investigate the type of dwarf mutation by light and dark morphological analysis,hormone sensitivity analysis and de-termination of endogenous hormonal content.The results showed that DW871 had a normal light-dark phenotype,and hypocotyles and stems were sensitive to exogenous strigolactone,gibberellin,and brassinolide,insensitive to ex-ogenous auxin,and the dwarf phenotype of DW871 could not be restored after bolting during external hormone ad-ministration.The determination of the endogenous hormones content showed that the auxin and gibberellin content in DW871 was higher than in HW871,and the content of strigolactones was lower than that in HW871,the brassino-lide content was no different from that in HW871.In conclusion,we speculated that the dwarf of Brassica napus DW871 could not be related to hormone defects.