Genetic analysis of nervonic acid,erucic acid and oleic acid content in seed of Brassica juncea
The beneficial fatty acids such as nervonic acid play an important role in human health.As a poten-tial source of nervonic acid,Brassica juncea is a functional oil crop with high quality.In order to investigate the ge-netic laws of important fatty acids such as nervonic acid in B.juncea,we created genetic analysis generations(P1,P2,F1/RF1,B1/RB1,B2/RB2,F2/RF2)with high nervonic acid germplasm JH1 and low nervonic acid germplasm JL4 of B.juncea as parents,and determined the contents of fatty acids in seeds of each generation.Correlation analysis and genetic analysis were carried out for the contents of nervonic acid,erucic acid and oleic acid.The results showed that oleic acid was significantly negatively correlated with erucic acid and nervonic acid(P<0.01),and nervonic ac-id was significantly positively correlated with erucic acid(P<0.01).The contents of nervonic acid,erucic acid and oleic acid in JH1 and JL4 were(2.998±0.274)%and(0.000±0.000)%,(47.644±2.343)%and(0.000±0.000)%,(8.853±1.963)%and(48.649±3.395)%,respectively,with significant differences between the parents.The contents of three fatty acids of F1 and RF1 were between those of the two parents and the phenotypes of isolated generations were widely distributed.Genetic analysis showed that the contents of the three acids had strong additive effect,and their best genetic model was MX2-ADI-ADI(two pairs of additive-dominant-epistatic major genes+additive-dominant-epistatic polygenes).The heredity of the three fatty acids in B2/RB2 and F2/RF2 was mainly regulated by the major genes,and the heritability was higher,with little environmental influence.Nervonic acid and erucic acid of B1 and RB1 were mainly inherited by major genes or polygenes,with little environmental influence,while oleic ac-id was more affected by environmental influence.The results of this study provided a theoretical basis for quality breeding of B.juncea with high nervonic acid.
Brassica junceanervonic aciderucic acidoleic acidmajor gene+polygenegenetic model
万方数据
维普
