Cloning and expression analysis of COP1 gene in peanut
To explore the sequence and expression pattern of COP1 gene in peanut,two COP1 genes were cloned from peanut,named as AhyCOP1a and AhyCOP1b,and the related analysis were conducted in this study.The results showed that AhyCOP1a and AhyCOP1b were 2103 bp and 2049 bp in length,respectively,and both of them contained 13 exons and 12 introns.The phylogenetic results showed that AhyCOP1a and AhyCOP1b grouped with COP1 genes in dicotyledon crops,and were closely related to AduCOP1 and AipCOP1,respectively.The re-sults indicated that the evolution of AhyCOP1a and AhyCOP1b originated from peanut wild accessions Arachis dura-nensis and A.ipaensis,respectively.In addition,AhyCOP1a and AhyCOP1b had 6 and 7 WD40 repeat motifs,re-spectively.The similarity of COP1 gene in N-terminus is low,and the homology of WD40 domain in C-terminus is high,indicating that WD40 domain is the main core functional region of COP1 gene.The tissue expression results showed that the expression pattern of AhyCOP1a and AhyCOP1b in the aboveground part was significantly higher than that in the underground part,and the highest expression was found in flowers,followed by leaves and stems.However,the expression level of AhyCOP1a in underground tissues is significantly higher than that of AhyCOP1b,suggesting that AhyCOP1a plays a more important role in the development of underground tissues.In addition,it was also found that light can promote the expression of COP1 gene in peanut,indicating that the expression of COP1 gene in peanut is significantly affected by light.In conclusion,the results of this study preliminarily clarified the sequence and expression pattern of COP1 gene in peanut,which laid a foundation for further exploration of the molecular function of COP1 gene.
万方数据
维普
