Interactive effect of N and Ca on the nitrogen metabolism enzyme activity,nitrogen utilization and calcium accumulation of peanut
In order to explore the effect of combined application of nitrogen and calcium on nitrogen utilization and calcium accumulation of peanut,a field experiment was conducted using Huayu 25 with two calcium(Ca)rates(0 and 600 kg·hm-2),and five nitrogen(N)rates(0,75,150,225,and 300 kg·hm-2)were set up in Jiyang and Yinmaquan experimental bases,respectively,to study the effects of nitrogen and calcium interaction on nitrogen me-tabolism enzyme,nitrogen utilization and calcium accumulation of peanut in different plots.The results showed that the interaction of nitrogen and calcium significantly increased the chlorophyll content of peanut in the early growth stage,at full fruit stage,under the condition of Ca0,the SPAD value of peanut with nitrogen application was 3.8%-14.8%higher than that without nitrogen application,while higher 3.9%-15.2%under the condition of Ca600.Ni-trogen fertilizer could increase the activity of nitrogen metabolizing enzyme in peanut.In the mature stage,the gluta-mine synthetase(GS)activity of Ca0 treatment under N300 condition was 57.8%higher than that of N0 condition,while that of Ca600 treatment was 87.3%higher.With the increase of nitrogen application rate,nitrogen use effi-ciency decreased gradually,while calcium fertilizer could improve peanut nitrogen use efficiency.Under the same nitrogen application conditions,nitrogen agronomic use efficiency and nitrogen use efficiency of Ca600 increased by 10.1%-24.6%、7.1%-48.7%and 2.1%-16.6%,respectively,compared with Ca0.The interaction of nitrogen and calcium increased the calcitonin distribution rate in mature pods.Under the same nitrogen application,the calcito-nin distribution rate in Ca600 treatment increased by 2.5-15.0 percentage points compared with Ca0 treatment,and the calcitonin distribution rate of N225 treatment was the highest.In summary,The interaction between nitrogen and calcium is beneficial to increase the content of chlorophyll in the early growth stage of peanut,increase the ac-tivity of nitrogen metabolic enzymes,improve nitrogen use efficiency and promote the distribution of calcitonin to pods,and the comprehensive performance of Ca600 and N225 treatments was the best.
万方数据
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