Establishment of a duplex PCR for differentially diagnosing of Cylindrocladium parasiticum and Neocosmospora vasinfecta
Cylindrocladium black rot and Neocosmospora foot rot are important diseases that seriously threaten healthy peanuts production.It is difficult to distinguish these two diseases due to their similar symptoms in the fields.In this study,duplex PCR detection was developed for the differential diagnosis of Cylindrocladium parasiti-cum and Neocosmospora vasinfecta based on calmodulin(cmdA)sequences.Two specific target bands(274 bp and 409 bp)were successfully amplified under the optimum conditions:reaction concentration of 1.8×,primer concen-tration of 0.2 μmol/L,annealing temperature of 62°C,and number of reaction cycles of 37.The detection limit of the duplex PCR system was 100 pg/μL The established duplex PCR assay was specific and efficient,could be used to distinguish accurately and control timely of the two confusing peanut diseases.
万方数据
维普
