The application of a Bacillus strain capable of degrading atrazine in sunflowers
A strain of Bacillus sp.ate-1 with high atrazine degrading ability was obtained by using SMS medi-um from the soil of maize field where atrazine had been applied for a long time.The strain was storage with number of CCTCC NO:M2019925.After characterization of the strain,it was found that the strain could tolerate atrazine at least 1000 μg/mL concentration and had the ability to produce IAA.The germination test of sunflower seed was car-ried out with the fermentation broth of the strain.It was confirmed that the dilution of fermentation broth from 40 to 100 times can significantly promote the root length during seed germination.By measuring the chlorophyll content of sunflower leaves in pot experiment,it was found that the chlorophyll content of sunflower increased in different degrees when the atrazine content was 5 mg/kg,10 mg/kg,15 mg/kg and 20 mg/kg soil,and the highest degree of im-provement was found in the soil treated with atrazine below 15 mg/kg.The results of the determination of soil Urease and Phosphatase also confirmed that the enzyme activity after adding degrading bacteria was higher than that with-out adding bacteria.The results provided a new microbial resource for the remediation of atrazine contaminated soil,and provided technical support for alleviating the damage of atrazine to sunflower seedlings.
万方数据
维普
