中国油料作物学报2024,Vol.46Issue(4) :930-937.DOI:10.19802/j.issn.1007-9084.2022362

一株降解阿特拉津的芽孢杆菌在向日葵中的应用

The application of a Bacillus strain capable of degrading atrazine in sunflowers

林志伟 白馨雨 吴占文 蔡杰 孙冬梅
中国油料作物学报2024,Vol.46Issue(4) :930-937.DOI:10.19802/j.issn.1007-9084.2022362

一株降解阿特拉津的芽孢杆菌在向日葵中的应用

The application of a Bacillus strain capable of degrading atrazine in sunflowers

林志伟 1白馨雨 1吴占文 1蔡杰 1孙冬梅1
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作者信息

  • 1. 黑龙江八一农垦大学,黑龙江 大庆,163000
  • 折叠

摘要

利用改良培养基SMS,从长期施用阿特拉津的玉米田土壤中分离获得了一株可高效降解阿特拉津的芽孢杆菌Bacillus sp.ate-1,菌株保藏号为:CCTCC NO:M2019925.该菌株可耐受至少1000 μg/mL浓度的阿特拉津,且具有产生吲哚乙酸(IAA)的能力.利用该菌株发酵液进行向日葵的萌发试验,证实发酵液稀释40到100倍对种子萌发过程中根长的促进作用显著;通过测定盆栽试验向日葵叶片的叶绿素含量发现:在阿特拉津含量为5、10、15和20 mg/kg土壤中添加降解菌后,向日葵的叶绿素含量均有不同程度提高,且以15 mg/kg的土壤处理提高程度最高;土壤脲酶与磷酸酶含量测定的结果也证实添加降解菌后酶活力均高于不加菌的处理.该结果的获得为阿特拉津污染土壤的修复提供了新的微生物资源,同时为缓解阿特拉津对向日葵幼苗危害提供技术支持.

Abstract

A strain of Bacillus sp.ate-1 with high atrazine degrading ability was obtained by using SMS medi-um from the soil of maize field where atrazine had been applied for a long time.The strain was storage with number of CCTCC NO:M2019925.After characterization of the strain,it was found that the strain could tolerate atrazine at least 1000 μg/mL concentration and had the ability to produce IAA.The germination test of sunflower seed was car-ried out with the fermentation broth of the strain.It was confirmed that the dilution of fermentation broth from 40 to 100 times can significantly promote the root length during seed germination.By measuring the chlorophyll content of sunflower leaves in pot experiment,it was found that the chlorophyll content of sunflower increased in different degrees when the atrazine content was 5 mg/kg,10 mg/kg,15 mg/kg and 20 mg/kg soil,and the highest degree of im-provement was found in the soil treated with atrazine below 15 mg/kg.The results of the determination of soil Urease and Phosphatase also confirmed that the enzyme activity after adding degrading bacteria was higher than that with-out adding bacteria.The results provided a new microbial resource for the remediation of atrazine contaminated soil,and provided technical support for alleviating the damage of atrazine to sunflower seedlings.

关键词

阿特拉津/降解/芽孢杆菌/IAA/向日葵

Key words

Atrazine/degradation/Bacillus/IAA/sunflower

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基金项目

中国科学院战略性先导科技专项(XDA28030203)

出版年

2024
中国油料作物学报
中国农业科学院油料作物研究所

中国油料作物学报

CSTPCDCSCD北大核心
影响因子:1.296
ISSN:1007-9084
参考文献量35
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