Regulation of shoot branching by BnaFZP in Brassica napus based on gene editing technology
The FRIZZYPANICLE(FZP)gene encodes the AP2/ERF transcription factor and is involved in plant architecture(shoot branching)regulation in the model plant Arabidopsis thaliana,which is an important target gene for the improvement of plant architecture in rapeseed.In the present study,we assessed the functions of rape-seed homologues of FZP gene in rapeseed at first time.According to the rapeseed bioinformatics analysis and gene cloning,we identified six copies of BnaFZP in Brassica napus genome.There is only an exon for all copies,which contains a conserved AP2-domain at N-terminal of their proteins.Gene expression analysis revealed that the BnaFZP expression levels were very low in different tissues,with a relative higher expression in roots,petals and si-liques.A total of 48 targeted mutants with loss-of-function alleles at different copies of BnaFZP gene were ob-tained at T0 generation using the CRISPR/Cas9 system and Agrobacterium-mediated transformation.The induced mutations were stably transmitted to successive generations,and a variety of BnaFZP homozygous T-DNA-free mu-tants were obtained in the T1 generation.Phenotypic observation of the obtained mutants showed that the homozy-gous BnaA02g35090D/BnaC02g08170D double mutants and homozygous BnaC03g09100D/BnaA02g35090D/BnaC02g08170D triple mutants presented significant much shoot branching,which indicted that BnaFZP is in-volved in the regulation of plant architecture.Collectively,the mutants generated in this study would provide valu-able resources for both basic studies and breeding programs.
万方数据
维普
