Mapping QTL for soybean branch number based on different genetic population
Branch number is an important agronomic trait of soybean,closely associated with the architecture,yield potential and adaptability.To mining the stable genetic loci of soybean branch number,148 recombinant in-bred lines(RILs)population crossed by Charleston(high-branched variety)and Dongnong 594(main stem variety),and 213 chromosome segment substitution lines(CSSL)population crossed by Suinong14(main stem variety)and ZYD00006(high-branched wild variety)were used as materials.With two genetic populations,we used inclusive composite interval mapping(ICIM)method in ICIMapping software and composite interval mapping(CIM)method in Win-QTL-Cart 2.5 software for mapping the QTLs of soybean branch number trait from 2018 to 2021,and the candidate genes from the QTL interval were screened.17 QTLs associated with branch number were detected in the RIL and CSSL population,among which qBNA2_1 and qBNK_1 were repeatedly detected on chromosome 8 and 9 in different years and population.According to gene annotation and qRT-PCR,Glyma.08G053700,Gly-ma.08G068200,Glyma.08G082400 and Glyma.09G167100 were predicted as candidate genes regulating soybean branch number from two stable QTLs interval.These results are helpful to understand the genetic mechanism of branch number formation and provide candidate genes and materials for studying ideal plant type of soybean.
万方数据
维普
