Genetic diversity analysis of vegetable soybean germplasm resources based on SSR markers
This study aimed to understand the genetic basis of different vegetable soybean germplasm resourc-es and the kinship of populations,212 pairs of SSR markers were selected to carry out genotypic analysis on 84 soy-bean germplasm materials from China(including Taiwan,Hubei,Jiangxi,Jiangsu,Liaoning and Anhui)and Japan.Furthermore,Cluster analysis was conducted according to Nei's genetic distance and unweighted matching method(UPGMA),And the hybrid model clustering method of structure software was used to infer the genetic structure of the material composition population.The results showed that the number of alleles amplified by SSR primers in the population material was 1-6,with an average of 2.08 per primer pair;Effective alleles range from 1-3.74,1.6 on av-erage;The average value of gene diversity is 0.29,and the average value of PIC was 0.24.The 84 materials were di-vided into 4 groups by cluster analysis.The results of cluster analysis were verified by two-dimensional and three-dimensional principal coordinate analysis diagrams of principal component analysis.The population structure analysis showed that when K=4,ΔK showed an obvious peak,indicating that the optimal number of groups was 4,which was basically consistent with the results of cluster analysis.The Q values of 74 of the 84 samples were higher than 0.7,indicating that the genetic diversity of vegetable soybean germplasm resources in this population was not rich enough and the genetic background was single.Therefore,we need to pay attention to the discovery and utiliza-tion of germplasm resources with large differences in genetic background and distant relatives,strengthen the shar-ing and exchange of germplasm materials,and promote the development of vegetable soybean breeding.
万方数据
维普
