Regulatory effect of soybean GmYUC12a on lateral root development and drought resistance in soybean
Lateral root(LR)plays an important role in plant drought resistance.This study was carried out to explore role of GmYUC12a in LR development under drought stress and lay a foundation for analysis of the response mechanism of soybean LR to drought stress.The changes of morphological characteristics,indole-3-acetic acid content and expression levels of several genes involved in IAA biosynthesis and signal transduction of roots and net photosynthetic rate(Pn)and relative water content(RWC)of leaves have been analyzed in Xudou 18 and Xu 9302 exposed to drought stress induced by 5%(m/V)polyethylene glycol 6000 treatment.Drought stressed improved LR number and length,total root length and IAA content,GmYUC12a,GmTIR1A and GmAFB3A expression levels in roots of Xu 9302,while the opposite effects on Xudou 18 were observed,which triggering a smaller drought-in-duced decrease in Pn and RWC in Xu 9302.The transgenic Xu 9302 hairy root complexes were generated by Agro-bacterium-mediated transformation method.Overexpression analyses showed that GmYUC12a caused an increase in LR number and length,total root length,IAA content and GmYUC12a,GmTIR1A and GmAFB3A expression levels in hairy roots and Pn and RWC in leaves of stressed Xu 9302 hairy root complexes.However,LR number and length did not change in stressed GmTIR1A-overexpressing and GmAFB3A-overexpressing hairy root complexes,as com-pared with stressed empty-vector hairy root complexes,while they increased in stressed complexes with hairy roots simultaneous overexpressing GmTIR1A and GmAFB3A.Thus,GmYUC12a up-regulated GmTIR1A and AFB3A which cooperated to induce LR development under drought stress,ultimately improving drought resistance in soy-bean.
万方数据
维普
