Establishment and application of a highly sensitive detection technique for di-butyl phthalate in edible oils
Edible oils are susceptible to contamination by di-butyl phthalate(DBP)during various stages of production,processing and storage.In this paper,we have developed and optimized a highly sensitive ELISA meth-od for the determination of di-butyl phthalate in edible oil using monoclonal antibodies as the recognition element.The results demostrated that the sensitivity(IC50)of the ELISA method was 16.29 ng/mL,and the detection range was 0.33～94.45 ng/mL(IC90～IC10)at an antigen encapsulation concentration of 1µg/mL,with 4%skimmed milk powder as the blocking agent,and the sample was used at pH 7.4 with an ionic concentration of 20 mmol/L in 40%methanol-PBS.The sensitivity(IC50)was 92.57 ng/mL,the detection range was 2.44-606.12 ng/mL(IC90-IC10),and the LOD(IC90)was 2.44 ng/mL.The matrix standard curve was established by the edible oil spiking test,and the sensitivity(IC50)was 92.57 ng/mL,the detection range was 2.44-606.12 ng/mL(IC90-IC10),and the LOD(IC90)was 2.44 ng/mL.The edible oil samples were spiked with DBP standards of 40 ng/mL,120 ng/mL and 200 ng/mL,re-spectively.The recoveries were 71.34%-97.23%,and the coefficients of variation were 1.05%-6.38%.The estab-lished DBP ELISA method was used to test the actual samples of soybean oil and corn oil,and the correlation be-tween the results and the national standard test method reached 99.17%,indicating that the method can be used for the determination of DBP content in the actual samples of edible oil.
万方数据
维普
