叶绿体相关突变体是研究光合作用、叶绿素生物合成、叶绿体结构发育等生理途径的优良遗传资源,同时作为标记性状在育种中具有一定的应用价值.本课题组发现一个来自恢复系轮回选择群体的子叶黄化致死突变体ytl(yellowing to lethal),突变体发芽出土后子叶一直处于黄化状态,播种9～15天后死亡,前期的研究将一个控制子叶黄化致死性状的位点定位到C09染色体上.表型观测显示,出苗7天后突变体与野生型相比株高与根长存在显著差异,明显偏短.遗传分析表明,该突变体由两对隐性核基因控制.利用单位点分离群体将BnaC02.YTL定位到对应ZS11参考基因组418 kb的物理区间内,结合定量分析与基因序列比对,BnaC02G0055700ZS作为候选基因的可能性较大.本研究为进一步精细定位基因BnaC02.YTL 及后续突变体的功能研究奠定了基础.
Genetic analysis of cotyledon yellow to lethal mutant BnaC02.YTL in Brassica napus
Chloroplast-related mutants are excellent genetic resources for studying physiological pathways such as photosynthesis,chlorophyll biosynthesis,and chloroplast structure development. They also have certain ap-plication values as marker traits in breeding. Our research team discovered a cotyledon yellowing to lethal mutant ytl (yellowing to lethal) from the recurrent selection population of restorer lines. The mutant remained yellowing state after germination and died after 9-15 days of sowing. Previous studies identified a locus trait of cotyledons on the C09 chromosome. Phenotypic observations showed that there were significant differences in plant height and root length between the mutant and the wild-type after 7 days of emergence,which were significantly shorter. Genet-ic analysis shows that the mutant is controlled by two pairs of recessive nuclear genes. Using unit point segregation population to locate BnaC02.YTL within the physical interval of 418 kb corresponding to the ZS11 reference ge-nome,combined with quantitative analysis and gene comparison sequencing,BnaC02G0055700ZS was identified as a highly likely candidate gene. This study lays the foundation for further precise mapping of the gene BnaC02.YTL and subsequent functional research of mutants.
万方数据
维普
