Gibberellin metabolism level and key gene transcription characteristics at seedling stage of Brassica napus L.
In order to understand the role of GA in heterosis of Brassica napus at seeding stage and effect of temperature on GA synthesis. In this study,the hybrid combination YG2009 × YC4 with strong yield advantage of hybrid F1 and its parents YG2009 and YC4 were selected as experimental materials,which grew at 5,15 and 25℃,respectively. The relationship between agronomic traits and total GA content of hybrid F1 and parents at 20 days of seedling age and the trend of total GA content with temperature were analyzed,and the relative transcription level of key enzyme genes in GA synthesis pathway was analyzed by qPCR. The results showed that temperature significant-ly affected the GA content in B. napus seedlings,which in turn affected the growth of seedlings. With the increase of temperature,the phenotypic traits such as plant height,leaf number,leaf width,leaf length,fresh weight and dry weight of different materials showed a significant upward trend. The total GA content in plants changed significantly with temperature. Canonical correlation analysis showed that phenotypic traits were positively correlated with the to-tal GA content,that is,the total GA content increased,which was beneficial to the growth of B. napus seedlings. The total GA content in YG2009 × YC4 plants increased with the increase of temperature,and reached the highest at 25℃. The total GA content in YG2009 × YC4 plants was 83.01％ higher than that of female parent YG2009 and 100.72％ higher than that of male parent YC4,reaching a significant difference level. At 25℃,the relative tran-scription level analysis of key enzyme genes of GA metabolism in different parts showed that there were different transcriptions of key enzyme genes in different parts of B. napus,resulting in a large difference in the total GA con-tent in each part. Grey correlation analysis showed that the relative transcription level of GA2ox6 had the highest grey correlation degree with the total GA content in roots,the relative transcription level of GID1 had the highest grey correlation degree with the total GA content in stems,and the relative transcription level of GA20ox4 had the highest grey correlation degree with the total GA content in leaves. It was speculated that the relative transcription levels of GA2ox6,GID1 and GA20ox4 had a great influence on the total GA content in plants.
万方数据
维普
