摘要
为丰富油茶根腐病生防资源并探究其抗病机理,采用平板对峙法和比色法等从油茶根系内生菌中筛选出拮抗促生细菌,并测定其对油茶根系防御酶活性及防御基因的影响.结果表明:结合拮抗效果、产IAA、溶有机磷、无机磷及产铁载体能力,从油茶根系筛选到两株高效内生拮抗促生菌AF9和AH6,经鉴定均为芽孢杆菌;二者均可以诱导油茶根系防御酶活性提高,超氧化物歧化酶(SOD)、苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)以及过氧化物酶(POD)活性分别在8 h、10 h、12 h和24 h达到峰值;PAL、RBOHC和POD基因分别在处理10 h、12 h及24 h时高度表达,对照组基因无明显表达.表明拮抗促生菌AF9和AH6可以诱导油茶产生系统抗性,为后期菌剂的研制以及油茶根腐病的生物防治奠定基础.
Abstract
In order to enrich the biocontrol resources of Camellia oleifera root rot disease and explore its resis-tance mechanism,antagonistic growth-promoting bacteria were screened from endophytic bacteria of C. oleifera root by plate confrontation method and colorimetric method,and their effects on defense enzyme activities and defense genes of C. oleifera root were measured. The results showed that two strains of efficient endophytic growth promoters AF9 and AH6 were screened from the root system of C. oleifera by combining the antagonistic effect,IAA produc-tion,organic phosphorus soluble,inorganic phosphorus production and iron-producing carrier capacity. Both strains were identified as Bacillus. Both of them could increase the root defense enzyme activities,and the activities of su-peroxide dismutase (SOD),phenylalanine ammoniase (PAL),polyphenol oxidase (PPO),and peroxidase (POD) reached the peak at 8 h,10 h,12 h,and 24 h,respectively. PAL,RBOHC and POD,genes were highly expressed at 10 h,12 h,and 24 h,respectively,while no significant expression was found in control group. The results indicated that the antagonistic growth promoting bacteria AF9 and AH6 could induce systemic resistance of C. oleifera,which laid the foundation for the development of later bacteriotics and biological control of C. oleifera root rot.
维普
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