Preparation of Trichoderma harzianum T-102 bactericide and its effects on peanut sheath blight and soil biodiversity
Peanut sheath blight caused by Rhizoctonia solani is an significant soil-borne disease in peanut pro-duction,which can seriously threaten the safety of peanut production and quality,and is the main obstacle factor in the sustainable and healthy development of peanut industry. In this study,the isolated Trichoderma harzianum strain T-102 with excellent control efficiency was cultured by submerged fermentation technology. Under the opti-mal conditions of 150 r/min,20℃ and the initial inoculation concentration of 7.5%,the water suspension of T. har-zianum T-102 with 105 cfu/mL was obtained. In Linyi city,Shandong province and Tangshan city,Hebei province,the water suspension of T. harzianum T-102 exhibited the great effect for controlling peanut sheath blight in the field. After spraying the water suspension of T. harzianum T-102,the disease index of peanut sheath blight was sig-nificantly reduced. Moreover,the yield of peanut could be increased by 41.2% and 31.7% in Linyi city and Tang-shan city,respectively. In addition,the water suspension treatment could change the structure and composition of microbial communities of peanut rhizosphere,and reduce the abundance of harmful microorganisms. Based on the above characteristics,the water suspension of T. harzianum T-102 is expected to carry out further commercial de-velopment and application.
peanut sheath blightRhizoctonia solaniTrichodermawater suspension concentratebiological control