Microfluidic paper-based analytical devices,as a low-cost and easy-to-operate detection platform for small amounts of liquid samples,have shown application potential in various fields,such as biochemical analysis,health monitoring,and environmental testing.With the rapid development of 3D printing technology,more and more researchers are using 3D printing technology for the fabrication and processing of microfluidic paper-based analytical devices.Herein,a review of 3D printing technology applied to paper microfluidics in recent years was provided,with a focus on the research progress in constructing hydrophobic barriers and structural/functional components via 3D printing technology.Also,future advances in 3D printing-based microfluidic paper-based analytical devices were prospected.
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