In order to determine whether spotted grass powder can be added to rabbit feed and the appropriate amount,in order to promote the development of unconventional feed resources,achieve localization of feed re-sources,and thereby reduce the cost of rabbit breeding feed.In this study,New Zealand white rabbits were select-ed,and a single factor randomized design was adopted.180 weaned rabbits aged 40±3 days were randomly as-signed to 6 groups,respectively,the amount of"West Sichuan"spot grass powder added:0%,6%,12%,18%,24%and 30%.The main nutritional indicators of each diet were basically the same.The pre feeding period was 7 days,and the feeding period was 28 days.During the test,the production performance and incidence rate were measured.After the test,slaughtering samples were taken,and the intestinal morphology,nutrient digestion Im-mune and microbiota related indicators.As a result,the addition of spotted grass powder did not cause a signifi-cant decrease in production performance(P＞0.05),while the 12%addition significantly increased the daily weight gain of rabbits(P＜0.05),increased the height of small intestinal villi(P＜0.05),and increased nutrient absorption area.However,it did not improve nutrient digestion efficiency,and there was no significant difference in the di-gestibility of protein and starch at the end of the ileum among the groups(P＞0.05);The addition of 12%spotted grass powder has the lowest health risk,and the incidence of diarrhea is only 3.3%.As the addition increases,the health risk increases,but there is no significant difference between the groups(P＞0.05).The addition of 30%can significantly reduce the vermiform index(P＜0.05),and the intestinal inflammatory factor IL-1 β And TNF-α The expression level of β-lactamase significantly increased(P＜0.05),and the intestine showed low degree of inflam-mation.Adding more than 24%of β-lactamase resulted in an increase in the activity of the cecal microbiota.In summary,the addition of spotted grass powder in the diet has no significant impact on production performance and health indicators.However,excessive addition can lead to increased health risks and feed formula costs.There-fore,the addition of spotted grass powder in the rabbit diet should not exceed 12%.
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