首页|白藜芦醇对AFB1致家兔脾脏和胸腺损伤的保护作用

白藜芦醇对AFB1致家兔脾脏和胸腺损伤的保护作用

扫码查看
为探究白藜芦醇(resveratroe)对黄曲霉毒素B1(AFB1)中毒家兔脾脏和胸腺的保护作用.将21只40日龄的家兔随机分成3组,分别为对照组、AFB1组和Res+AFB1组,对照组饲喂基础饲粮,AFB1组饲喂添加0.3 mg/kg AFB1的饲粮,Res+AFB1组饲喂含有0.3 mg/kg AFB1以及30 mg/kg Res的饲粮,试验持续21 d.检测血浆免疫细胞数量,取出脾脏和胸腺组织制作石蜡切片,进行HE和PAS染色观察,并对脾脏和胸腺组织中抗氧化指标进行测定.结果显示,Res可显著提高AFB1损伤兔脾脏和胸腺中GSH-Px、SOD、T-AOC(P<0.05),显著降低MDA含量(P<0.05),恢复脾脏和胸腺组织结构.此外,Res可恢复AFB1诱导的白细胞、淋巴细胞、中性粒细胞及单核细胞其数量变化,恢复机体免疫功能.结果表明30 mg/kg Res可缓解AFB1诱导的家兔脾脏和胸腺组织结构损伤,增强抗氧化能力并改善家兔免疫功能.
Protective Effect of Resveratrol on AFB1 Induced Damage to Spleen and Thymus of Rabbits
This study was to explore the protective effects of resveratrol(Res)on the spleen and thymus of rabbits with aflatoxin B1(AFB1)toxicity.Twenty-one 40-day-old rabbits were randomly divided into 3 groups:control group,AFB1,and Res+AFB1.The control group was fed a basal diet,the AFB1 group was fed a diet containing 0.3 mg/kg AFB1,and the Res+AFB1 group was fed a diet containing 0.3 mg/kg AFB1 and 30 mg/kg Res for 21 days.The numbers of immune cells in plasma were detected.Spleen and thymus tissues were collected to make paraffin sections for H.E.and PAS staining observation.Antioxidant indexes in spleen and thymus tissues were al-so determined.The results showed that Res significantly increased GSH-Px,SOD,T-AOC(P<0.05)and de-creased MDA(P<0.05)in the spleen and thymus of AFB1-damaged rabbits,and restored the tissue structures.Additionally,Res reversed the AFB1-induced changes in white blood cell,lymphocyte,neutrophil and monocyte counts,restoring immune function.These findings indicate that 30 mg/kg Res can alleviate AFB1-induced struc-tural damage in rabbit spleen and thymus,enhance antioxidant capacity and improve immune function.

resveratrolAFB1spleenthymusoxidative stressimmune function

常陈昊、刘玉梅、蔺丽丽、赵娅楠、张自强

展开 >

河南科技大学动物科技学院,洛阳 471000

白藜芦醇 AFB1 脾脏 胸腺 氧化应激 免疫功能

河南省重点研发与推广专项校企合作项目

202102110093横20210018

2024

中国养兔
江苏省畜牧总站,中国畜牧业协会兔业分会,江苏畜牧兽医职业技术学院

中国养兔

影响因子:0.171
ISSN:1005-6327
年,卷(期):2024.(3)
  • 5