Changes of Intestinal Microflora in Rabbit Infected with Eimeria Stiedai
In this study,the changes of the gut microbiota in rabbits induced by infection with Eimeria stiedai(E.stiedai).Twelve SPF female New Zealand white rabbits were randomly divided into the infection group(G3)and control group(C3).In the infection group,rabbits received 1.0×105 sporulated E.stiedai orally,while those in control group were given equivalent normal saline only.Rabbits were sacrificed at 23 PI(day post-infection),then their small intestine contents were collected to extracte DNA.The composition of gut microbiota was analyzed by sequencing of 16S rRNA and bioinformatics methods.Our results showed that there was no significant difference in the alpha diversity of the intestinal microbiota between the control and infected rabbits,while the beta diversity was changed,which indicating that the two groups had different microbiota structures.Metastats analysis showed that the abundance of Bacteroides,Parabacteroides,Subdoligranulum and Lachnoclostridium producing butyrate were significantly(P<0.05)increased in intestine of rabbits infected with E.stiedai,compared with C3 group.How-ever,the abundance of Paenibacillus were significantly(P<0.05)decreased.Moreover,LEfSe analysis showed that the abundance of Bacteroides was significantly increased(P<0.05).In conclusion,the composition of gut microbio-ta in rabbits infected with E.stiedai is significantly different from that of normal rabbits.
Eimeria stiedaiNew Zealand white rabbitsgut microbiota
NETL
NSTL
万方数据
