金耳总黄酮的体外抗氧化作用
Antioxidant Effect of Total Flavonoids of Auriculus Auriculus in Vitro
殷金玲 1马亮2
作者信息
- 1. 吉林磐石经济开发区投资促进局,吉林吉林 132300
- 2. 吉林省维伊康生物科技有限公司,吉林吉林 132300
- 折叠
摘要
目的 探讨食药用菌新品种金耳在微波萃取条件下其总黄酮的体外抗氧化作用.方法 采用分光光度法分别测定了金耳总黄酮对羟基自由基、超氧阴离子自由基、DPPH自由基的清除能力及相对还原能力,同时与维生素C(VC)进行了对比.结果 在0.14 mg/ml浓度时,金耳总黄酮对羟基自由基的清除率为84%.在0.07 mg/ml浓度时,其对超氧阴离子自由基的清除率为91%,在0.12 mg/ml浓度时,其对DPPH自由基的清除率为90%,同时金耳总黄酮还有较强的还原能力.结论 金耳总黄酮在一定浓度范围内具有良好的体外抗氧化作用.
Abstract
Objective To explore the in vitro antioxidant effect of total flavonoids of the new edible and medicinal fungus Aurantium aurantium under microwave extraction.Methods The scavenging ability to hydroxyl radical,superoxide anion radical and DPPH radical,and relative reducing ability of total flavonoids were determined by spectrophotometry,and compared with vitamin C(VC).Results At 0.14 mg/ml,the scavenging rate of hydroxyl radical was 84%.At the concentration of 0.07 mg/ml,the scavenging rate of superoxide anion free radical was 91%,and at the concentration of 0.12 mg/ml,the scavenging rate of DPPH free radical was 90%,and the total flavonoids of Aurantium aurantium also had a strong reducing ability.Conclusion The total flavonoids of auriculus auriculus have a good antioxidant effect in vitro within a certain concentration range.
关键词
金耳/总黄酮/抗氧化/分光光度法Key words
Aurantium aurantium/Total flavonoids/Antioxidation/Spectrophotometry引用本文复制引用
出版年
2024