气相色谱-质谱联用法检测可疑槟榔中非法添加的替来他明
Determination of tiletamine in areca nut of illegally added by gas chromatography-mass spectrometry
宋辉 1李想 1许英健 1邢丽梅 1王千羽 1杜瑞轩1
作者信息
- 1. 中国刑事警察学院刑事科学技术学院,沈阳 110854
- 折叠
摘要
目的 针对植物样本中非法添加的处方类药物替来他明(tiletamine),建立气相色谱-质谱联用(GC/MS)定性、定量分析方法,并进行方法学评价.方法 公安机关收缴的槟榔植物样本研磨粉碎后经甲醇溶剂超声提取,提取液进行气相色谱-质谱联用分析,选用HP-5 MS(30m × 0.25 mm × 0.25 pm)色谱柱,对提取条件进行了优化.结果 此方法在0.5～500 μg/g范围内线性关系良好,相关系数R2=0.9997;检出限LOD=0.15 μg/mL,定量限LOQ=0.5 μg/mL;方法 回收率78.8％～96.5％,RSD值≤7.6％.结论 所建方法简便快捷,灵敏度高,稳定性好,适用于法庭科学中该类案件检验工作.
Abstract
Objective In response to the abuse of the controlled drug tiletamine,which is illegally added into the plant samples,gas chromatography-mass spectrometry(GC-MS)method for qualitative and quantitative detection were established and the methodology was evaluated.Methods Areca nut samples confiscated by public security organs were pulverized and extracted by ultrasonic extraction with methanol.HP-5MS(30m × 0.25mm × 0.25um)column was selected,and the extraction conditions were optimized.Results The linear range was 0.5～500.0 µg/g with correlation coefficient of 0.9997.The limit of detection(LOD)was 0.15 μg/mL and the limit of quantitation(LOQ)was 0.5 μg/mL.The recoveries were in the range of 78.8％～96.5％.The relative standard deviations(RSDs)was less than 7.6％.Conclusion The established method is simple,rapid,sensitive and stable,is suitable for the examination of such cases in court science.
关键词
气相色谱-质谱联用/槟榔/替来他明/超声提取Key words
GC-MS/areca nut/tiletamine/ultrasound extraction引用本文复制引用
基金项目
辽宁省教育厅科学研究经费重点项目(LJKZ0071)
出版年
2024
NETL
NSTL
万方数据