LC-MS/MS对毛发中富马酸喹硫平的分段检测
Segmented detection of quetiapine fumarate in hairs by LC-MS/MS
彭俞霖 1张婷 1佟澳 1许英健 1吴发龙 1王嘉明 1陈旭潮1
作者信息
- 1. 中国刑事警察学院刑事科学技术学院,沈阳 110031
- 折叠
摘要
目的 建立一种高效液相色谱-质谱联用(LC-MS/MS)法,对毛发中的喹硫平进行分段含量测定.方法 毛发样品经过冷冻研磨为粉末后用甲醇超声提取30 min,经0.22 μm滤膜.色谱柱使用Thermo Gold ODS C18(150 mm×2.1 mm,5 μm),流动相为甲醇-0.2％冰乙酸水溶液,质谱采用ESI+离子源,MRM多反应监测.结果 喹硫平在0.15 ng/mg～50 ng/mg范围内峰面积与浓度呈良好的线性关系,喹硫平的工作曲线方程为Y=1.1046X-0.9789(R2=0.9934),方法绝对回收率在83.1％～102.6％.结论 建立了分段测定毛发中喹硫平含量的LC-MS/MS法,该方法结果灵敏、准确、特异性强,在药物滥用致死的刑事案件中具有一定应用价值.
Abstract
Objective To establish a high-performance liquid chromatography-mass spectrometry(LC-MS/MS)method for the segmented determination of quetiapine in hair.Methods The hair samples were frozen and ground into power,then extracted with methanol ultrasound for 30 minutes and filtered through a 0.22 μm membrane.The chromatographic column was Thermo Gold ODS C18(150 mm ×2.1 mm,5 μm),and the mobile phase was methanol-0.2％aqueous glacial acetic acid.Mass spectrometry was performed with ESI+ion source and MRM multiple reaction monitoring.Results The peak area of quetiapine showed a good linear relationship with concentration in the range of 0.15 ng/mg to 50 ng/mg.The operating curve equation of quetiapine is Y=1.1046X-0.9789(R2=0.9934).The absolute recovery of the LC-MS/MS method was in the range of 83.1％to 102.6％.Conclusion A LC-MS/MS method for segmented determination of quetiapine content in hair has been established,which is sensitive,accurate,and highly specific.This method has certain application value in criminal cases of drug abuse causing death.
关键词
毛发/喹硫平/分段检验/LC-MS/MSKey words
hair/quetiapine/segmentation test/LC-MS/MS引用本文复制引用
出版年
2024
NETL
NSTL
万方数据