Objective To establish a multicomponent quantification method for Guli capsules and explore the application of chemometrics and grey relational analysis(GRA)in the quality evaluation of them.Methods Samples were gradiently eluted with acetonitrile-0.2%acetic acid as the mobile phase based on high-performance liquid chromatography(HPLC)method.The detection wavelengths were 270,430 and 250 nm.A multicomponent quantitative analysis mode was established with puerarin as the internal standard.The comprehensive quality of Guli capsules was evaluated by chemometrics and GRA.Results The results of methodological verification of external standard method(ESM)met the requirements.There was no significant difference between the quantitative results obtained by relative correction factor method and ESM.Chemometrics analysis showed that icariin,puerarin,epimedin,psoralen and curcumin were the representative differential components of Guli capsules.The GRA model was stable,and the relative correlation was from 0.309 5 to 0.614 5.Conclusion The established method wis simple and practical,and chemometrics and GRA could be used to evaluate the overall quality of Guli capsules.
关键词
骨力胶囊/一测多评法/灰色关联度分析法/化学计量学/综合评价
Key words
Guli capsules/Quantitative analysis of multi-components by a single marker/Grey relational analysis/Chemometrics/Comprehensive evaluation
维普
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