首页|高效液相色谱-紫外检测法同时测定类鼻疽患者血浆中亚胺培南、美罗培南和头孢他啶的浓度

高效液相色谱-紫外检测法同时测定类鼻疽患者血浆中亚胺培南、美罗培南和头孢他啶的浓度

Simultaneous determination of the plasma concentrations of imipenem,meropenem,and ceftazidime by HPLC-UV method in patients with melioidosis

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目的 建立一种快速、经济的高效液相色谱-紫外检测法(HPLC-UV),用于同时测定人血浆中亚胺培南、美罗培南和头孢他啶的药物浓度.方法 采用Millipore 10KD超滤离心去蛋白,使用互为内标法,色谱柱为SunFire-C18 柱(4.6 mm×250 mm,5 µm);预柱为 C18柱(4.0 mm×2.1 mm,5 µm),流动相为0.1 mol·L-1的3-(N-吗啡啉)丙磺酸(pH = 7.0)和乙腈,梯度洗脱,检测波长299 nm,流速1.0 mL·min-1,运行时间30 min,进样体积30 µL.结果 亚胺培南、美罗培南和头孢他啶的保留时间分别为6.699 min、10.795 min和8.722 min.血浆内源性物质对样品的测定无干扰,峰形良好.该方法具有良好的线性、准确度和精密度.不同浓度的样品在-20℃反复冻融、-20℃长期冷冻和4℃(48 h)下均表现出了较高的稳定性.结论 该方法操作成本低、专属性强、分离效果好,可用于类鼻疽患者血浆中亚胺培南、美罗培南和头孢他啶治疗药物浓度的测定.
Objective To develop a rapid and cost-effective method of high performance liquid chromatography-ultraviolet detection(HPLC-UV)for the simultaneous determination of the plasma concentrations of imipenem,meropenem and ceftazidime in human plasma.Methods The protein was removed by Millipore 10KD ultrafiltration centrifugation,and the chromatographic column was SunFire C18 column(4.6 mm×250 mm,5μm).The pre-column was C18 column(4.0 mm×2.1 mm,5μm),and the mobile phase was 0.1 mol·L-1 3-(N-morpholino)propanesulfonic acid and acetonitrile with pH 7.0.Samples were gradiently eluted with the flow rate of 1.0 mL·min-1 for 30 minutes and detected at the wavelength of 299 nm.The injection volume was 30μL.Results The retention time of imipenem,meropenem and ceftazidime were 6.699 min,10.795 min and 8.722 min,respectively.The peak form was pleasing without disturbance of the endogenous components in plasma.The method showed good accuracy,precision,and linear relationship.The samples with different concentrations showed high stability at-20℃ and 4℃.Conclusion The method has low operation cost,strong specificity and good separation effect,which can be used for routine determination of the plasma concentrations of imipenem,meropenem and ceftazidime in patients with melioidosis.

MelioidosisImipenemMeropenemCeftazidimeHigh performance liquid chromatography-ultraviolet detection

符香香、钟莉莉、陈君、柴芳、云雄、吴华、王敏

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海南医学院药学院,海口 571199

海南省人民医院(海南医学院附属海南医院)药学部,海口 570311

三亚市人民医院药学部,三亚 572000

海南省人民医院(海南医学院附属海南医院)检验科,海口 570311

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类鼻疽 亚胺培南 美罗培南 头孢他啶 高效液相色谱-紫外检测法

三亚市高等学校及医疗机构专项科技计划海南省重点研发计划海南省重点研发计划海南省自然科学基金面上项目海南省卫生健康行业科研项目

2021 GXYL14ZDYF2022SHFZ050ZDYF 2019141821MS13022A200229

2024

10.3969/j.issn.1672-8157.2024.01.009

中国药物应用与监测
中国人民解放军总医院

中国药物应用与监测

CSTPCD
影响因子：1.983
ISSN：1672-8157
年,卷(期)：2024.21(1)
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