Objective To research the protective effect and mechanism of extract of ginkgo biloba(EGB)injection on noise induced hearing loss(NIHL)rats by regulating phosphatidylinositol 3-kinase(PI3K)/mitogen activated protein kinase(MAPK)signaling pathway.Methods Sprague dawley(SD)rats were randomly divided into control group,NIHL model group,low-dose EGB group(EGB-L group,0.7 mg·kg-1),high-dose EGB group(EGB-H group,1.4 mg·kg-1),and high-dose EGB + 740Y-P group(EGB-H + 740Y-P group,1.4 mg·kg-1 EGB + 0.02 mg·kg-1 740Y-P).Auditory brainstem response(ABR)was used to evaluate the hearing of rats in each group.Hematoxylin eosin(HE)staining was used to observe the histopathological changes of rat cochlea tissue and the number of spiral ganglion cells.The mRNA expression of PI3K and MAPK in rat cochlea tissue was detected by reverse transcription quantitative polymerase chain reaction(RT-qPCR).Western blot method was used to detect the related protein expression on PI3K/MAPK signaling pathway in rat cochlea tissue.The proliferative activity of cochlear hair cells was determined by cell counting kit-8(CCK-8)method.Results Compared with the control group,the hearing threshold of each ear at 2,4 and 8 kHz,the mRNA expression of PI3K and MAPK in cochlea tissue,the phosphorylation level of MAPK,PI3K and protein kinase B(AKT)in cochlea tissue in the model group significantly increased(P<0.05),and the number of spiral ganglion cells and hair cells in cochlea tissue significantly decreased(P<0.05).Compared with the model group,the changes of the same indicators in the EGB-H group were contrary to those above(P<0.05).740Y-P,a PI3K activator,reduced the protective effect of EGB on NIHL rats.Conclusion EGB might protect NIHL rats by down-regulating PI3K/MAPK signaling pathway.
Noise induced hearing lossInjection of extract of ginkgo bilobaPhosphatidylinositol 3-kinase/mitogen activated protein kinase signaling pathway
维普
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