Therapeutic efficacy of Melastoma sanguineum Sims in wound healing and its mechanism prediction
Objective To explore the therapeutic effect of Melastoma sanguineum Sims,a traditional Chinese medicine,on wound healing and explore its potential mechanism.Methods The crude liquid of Melastoma sanguineum Sims was obtained by drug decoction method,and it was diluted by 50 times,100 times,200 times and 400 times to act on human keratinocytes HaCaT cultured in vitro.The study included the control(CON)group and experimental(M50,M100,M200,M400)group.The cell viability was detected by CCK8 method,and the wound healing effect tested by scratch model.The metabolites of Melastoma sanguineum Sims were analyzed by liquid chromatography-mass spectrometry and a drug-disease-target interaction network was constructed to predict the mechanism of intervention of the drug in wound healing.Results As compared with the control group,the relative proliferation rates of HaCaT cells in M50,M100,M200 and M400 groups((112.59±2.04)%,(119.04±2.15)%,(128.40±1.10)%,(133.72±2.83)%)were significantly increased(P<0.05)in a concentration-dependent manner.The scratch healing rate of M400 group at 12 h and 24 h((86.93±4.90)%,(95.13±3.70)%)was higher than that of the control((36.29±2.97)%,(85.01±2.10)%),demonstrating statistically significant difference(P<0.05).A total of 539 effective active components were screened by non-targeted metabolomics and 592 targets of active components intersected with wound healing.The enrichment analysis showed that the intersection targets were mainly involved in 2 655 biological processes,enriched in 92 cell components,mainly related to 164 molecular functions and 180 signaling pathways.Conclusion The traditional Chinese medicine,Melastoma sanguineum Sims,is expected to become an effective drug for the treatment of trauma because it can promote the proliferation of HaCaT cells and the healing after scratch injury.The drug might be used to treat trauma by acting on TNF,SRC,TP53 and other targets through active ingredients such as glyceryl linolenate,betulin,L-tryptophan,morin,etc.The potential mechanism of which may be related to the PI3K-Akt signaling pathway.
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