首页|基于血清药物化学和网络药理学的荜茇抗胃溃疡药效物质基础分析

基于血清药物化学和网络药理学的荜茇抗胃溃疡药效物质基础分析

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荜茇(PL)是具有药食两用性的常用中药,中医临床多生用,具有温中散寒、行气止痛的功效,用于胃脘冷痛、胃寒呕吐等,同时也是蒙医、藏医和印度传统常用药物.在本研究中,首先建立大鼠胃溃疡动物模型,考察荜茇对胃粘膜的保护作用,进而将血清药物化学与网络药理学相结合,挖掘荜茇药效物质基础,从荜茇中筛选药效物质和潜在作用靶点.研究结果表明,荜茇对乙醇诱导的胃溃疡具有显著的保护作用;网络药理学预测了潜在的药物-疾病共同靶点后,GO富集分析预测荜茇可能影响多种生物过程,如脂质代谢和炎症反应.KEGG富集分析提示TNF和AGE-RAGE信号通路可能参与其中,分子对接预测芝麻素与胡椒碱等可能是荜茇抗炎和抗菌作用的主要成分.此外,荜茇的血清药物化学揭示了入血成分中有23种原型成分和17种代谢物.本研究整合了血清药物化学和网络药理学,为荜茇在胃溃疡治疗中的药效物质研究提供了实验依据.
Integrated serum pharmacochemistry and network pharmacology analyses reveal the bioactive metabolites and potential functional mechanism of Piper longum L.in the treatment of gastric ulcer in rats
The fruit of Piper longum L.(PL)is widely utilized in China for medicinal and dietary purposes,and it is also used in Ayurvedic medicine in India to treat gastric disorders and other ailments.In the present study,a strategy combining serum pharmacochemistry with network pharmacology was proposed to screen the bioactive metabolites derived from PL and explore their potential functional mechanisms.The strategy primarily involved investigating the anti-gastric ulcer activities,network pharmacology analysis,and metabolite identification.The results demonstrated the efficacy of PL in protecting against ethanol-induced gastric ulcers.Through network pharmacology approaches,potential drug-disease common targets were identified.GO enrichment analysis predicted that PL might influence multiple biological processes,such as lipid metabolism and inflammatory response.KEGG enrichment analysis suggested the involvement of the TNF and AGE-RAGE signaling pathways.Molecular docking further indicated that the synergistic effect of sesamin,piperine,and other ingredients could be the main contributors to PL's anti-inflammatory and antibacterial activities.Additionally,the serum pharmacochemistry of PL was established,enabling the screening of absorbed components and metabolites for their anti-gastric ulcer activity.A total of 23 prototypes and 17 metabolites were found to significantly contribute to the anti-gastric ulcer activity,suggesting that these bioactive compounds could serve as potential active components of PL.Overall,this investigation provided an experimental foundation to support the clinical application of PL in gastric ulcer therapy.

Serum pharmacochemistryNetwork pharmacologyMetabolitePiper longum L.Gastric ulcer

张弘、张莎莎、王焕芸、梁越、武世奎、孙丽君、夏慧敏、白云霞、张慧文

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内蒙古医科大学药学院,内蒙古呼和浩特 010110

鄂尔多斯市中心医院中心实验室,内蒙古鄂尔多斯 017000

内蒙古自治区人民医院,内蒙古呼和浩特 010017

包头医学院,内蒙古包头 014040

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血清药物化学 网络药理学 代谢物 荜茇 胃溃疡

内蒙古自治区自然科学基金Mongolian Medicine Synergy Innovation Center Scientific Research Foundation of Inner MongoliaKey Technology Project Foundation of Inner MongoliaPh.D.Fund Project of Inner Mongolia Medical University

2022MS08023MYYXTYB2021082021GG0176YKD2020BSJJ014

2024

10.5246/jcps.2024.02.013

中国药学(英文版)
中国药学会

中国药学(英文版)

CSTPCD
影响因子：0.534
ISSN：1003-1057
年,卷(期)：2024.33(2)
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