GAS5,as the ceRNA of miR-223-3p,mediates pyroptosis and inhibits the proliferation of brain glial cells U87,and quercetin can enhance its effect
This study explored the inhibitory effect of miR-223-3p on the proliferation of brain glioma cells and its potential mechanism in relation to growth arrest-specific transcripts(GAS5).We utilized the StarBase tool to predict the binding of GAS5 to miR-223-3p,further confirming the binding relationship between GAS5 and miR-223-3p through a dual luciferase reporter assay and RNA immunoprecipitation.In vitro cultured U87 brain glial cells were categorized into four groups:the control group,quercetin group,GAS5 inhibition group,and quercetin+si-GAS5 group.We analyzed and compared the impacts of quercetin on GAS5/miR-223-3p and pyroptotic proteins(NLRP3,ASC,caspase-1,GSDMD,and GSDME),as well as the proliferative capacity of U87 cells.StarBase's predictions indicated that GAS5 bound to miR-223-3p.This was corroborated by the dual luciferase reporter assay and RNA immunoprecipitation assay,which demonstrated a direct binding between GAS5 and miR-223-3p.Quercetin was observed to enhance GAS5 expression,inhibit the expression of miR-223-3p and pyroptotic proteins(NLRP3,ASC,caspase-1,GSDMD,and GSDME),and suppress the proliferation and invasion of U87 cells(P＜0.05).Downregulation of GAS5 expression correlated with an increase in the expression of miR-223-3p and pyroptotic proteins(NLRP3,ASC,caspase-1,GSDMD,and GSDME),promoting the proliferation and invasion of RC-4B/C cells(P＜0.05).Quercetin was able to reverse the increase in miR-223-3p expression and pyroptotic proteins(NLRP3,ASC,caspase-1,GSDMD,and GSDME)induced by the downregulation of GAS5,thereby inhibiting the proliferation and invasion of U87 cells(P＜0.05).In conclusion,quercetin might inhibit the proliferation of U87 brain glioma cells by promoting the expression of GAS5,which in turn competitively inhibited miR-223-3p and suppressed the pyroptotic signaling pathway.
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