首页|基于网络药理学探讨复方苦参注射液治疗黑色素瘤的作用机制

基于网络药理学探讨复方苦参注射液治疗黑色素瘤的作用机制

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本文通过网络药理学研究复方苦参注射液对防治黑色素瘤的作用机理.运用TCMSP数据库探索复方苦参注射液中苦参和土茯苓的活性成分,通过Pubchem和SwissTargetPrediction数据库检测苦参和土茯苓的药物靶点,经GeneCards、OMIM、TTD和CTD数据库检索与黑色素瘤相关的疾病靶点,并运用Cytoscape 3.9.1建立中药-成分-疾病-靶点网络,使用String与Cytoscape建立交集靶点蛋白的相互作用网络,经Cyto-Hubba插件根据MCC算法进行拓扑分析,筛选关键靶点.最后,使用Metascape数据库对相关靶点进行GO和KEGG分析.最终在复方苦参注射液中共筛选出60个活性成分,包括槲皮素、去甲果肉皮素、白军恩等;与黑色素肿瘤相关的靶点有916个,包括AKT1、EGFR、ESR1等.此外,复方苦参注射液的抗黑色素瘤功能包括对磷酸化效应的调节和影响氧化应激反应等生物学过程,并通过调节癌症通道、内分泌通路、Th17细胞分化通道等信息通路发挥抗肿瘤效果.本研究首次报道了复方苦参注射液治疗黑色素瘤疾病的潜在作用机制,为后续实验研究提供了思路与依据.
Unraveling the mechanisms of compound Sophora flavescens injection in melanoma treatment:A network pharmacology
In this paper,the mechanism of effect of compound Sophora flavescens injection on the prevention and treatment of melanoma was studied through network pharmacology.TCMSP database was used to explore the active ingredients of Sophora flavescens and Poria cocos in compound Sophora flavescens injection,the drug targets of Sophora flavescens and Poria cocos were detected by Pubchem and Swiss Target Prediction databases,the disease targets related to melanoma were retrieved through GeneCards,OMIM,TTD and CTD databases,and Cytoscape 3.9.1 was used to establish a TCM-component-disease-target network.The interaction network of intersecting target proteins was established by String and Cytoscape,and the topological analysis was performed according to the MCC algorithm by the Cyto-Hubba plug-in to screen the key targets.Finally,GO and KEGG analyses of the relevant targets were performed using the Metascape database.Finally,a total of 60 active ingredients were screened in the compound Sophora flavescens injection,including quercetin,norcetin and white junen.There are 916 targets related to melanoma tumors,including AKT1,EGFR,ESR1,etc.In addition,the anti-melanoma functions of Sophora flavescens injection include the regulation of phosphorylation effects and the effects of oxidative stress responses,and exert anti-tumor effects by regulating cancer pathways,endocrine pathways,Th17 cell differentiation channels and other information pathways.This study reported for the first time the potential mechanism of compound Sophora flavescens injection in the treatment of melanoma disease,and provided ideas and basis for follow-up experimental research.

Compound Sophora flavescens injectionMelanomaNetwork pharmacologyMechanism of action

崔旭阳、姬中杰、时潘扬、魏晓岑、马玉宁、邢梦真

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山东中医药大学医学院,山东济南 250355

山东中医药大学针灸推拿学院,山东济南 250355

山东中医药大学药物研究院,山东济南 250355

复方苦参注射液 黑色素瘤 网络药理学 作用机制

Shandong Province Postdoctoral Innovation Talent Support Program ProjectOpen Project of Key Laboratory of Photochemical Conversion and Functional Materials,Chinese Academy of Sciences

SDBX2022018PCOM202317

2024

10.5246/jcps.2024.05.034

中国药学(英文版)
中国药学会

中国药学(英文版)

CSTPCD
影响因子：0.534
ISSN：1003-1057
年,卷(期)：2024.33(5)
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