Quantification of eight components in Paeonia lactiflora and Glycyrrhiza decoction using UHPLC-MS/MS
This study aimed to utilize ultra-high-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)to analyze and quantify eight therapeutic components in Paeonia lactiflora and Glycyrrhiza decoction.The goal was to establish an accurate,efficient,and applicable method for determining the composition of this formula,providing a reference for its clinical application.Methanol was employed to extract the therapeutic components from Paeonia lactiflora and Glycyrrhiza decoction,forming the test sample solution.UHPLC-MS/MS technology was applied to separate and analyze the test samples.The results indicated that upon analysis,the eight reference standards exhibited excellent linear relationships,and the test samples remained stable within 24 h.The content of paeoniflorin,paeonolide,glycyrrhizic acid A,quercetin,chebulic acid,liquiritin,naringenin,and glycyrrhetic acid was determined to be 3.530±0.124,0.139±0.006,0.245±0.008,0.779±0.026,0.433±0.021,0.388±0.018,0.617±0.013,and 0.081±0.005 mg/mL,respectively.This study established that UHPLC-MS/MS analysis demonstrated high efficiency and broad applicability,reflecting the chemical composition characteristics of Paeonia lactiflora and Glycyrrhiza decoction.This research provided a quantitative analysis method for the eight therapeutic components in Paeonia lactiflora and Glycyrrhiza decoction,applicable to its clinical use and quality control.
Paeonia lactiflora and Glycyrrhiza decoctionUHPLC-MS/MSMedicinal compositionContent determination
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