Synthesis of mesoporous sodium hyaluronate microspheres via electrostatic spray method
In the present study,we employed electrostatic spray technology to fabricate mesoporous microspheres containing sodium hyaluronate(SHMM).This approach aimed to overcome a significant challenge in the formulation development process,specifically addressing the limited water-locking ability inherent to the double-helix structure of sodium hyaluronate(SH).Various parameters were systematically investigated,including the solvent employed,concentrations of SH and poly(ethylene oxide),electrospray flow rate,voltage settings,and needle diameter.Through systematic single-factor testing,we identified the optimal formulation process for generating SH microspheres characterized by favorable morphology and particle size.Additionally,a similar single-factor examination focused on the concentration of the pore-forming agent and the drying temperature,leading to the successful production of mesoporous microspheres with discernible pores.The porosity of the three distinct batches of mesoporous microspheres was consistently measured at 20.30％±1.51％.Moreover,all these microspheres displayed a negative surface potential when suspended in water,affirming their strong capacity to bind with positively charged protein drugs.These findings underscored the feasibility of drug loading through ion exchange in subsequent stages.Notably,the successful preparation of recombinant human interferon α-2b crosslinked mesoporous microspheres(rhIFN α-2b-SHCMM)was achieved,demonstrating both high entrapment efficiency and an enhanced drug loading capacity.
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