Objective To study the characteristics of vaginal microflora in women with different degree of cervical lesions,and to explore the possibility of preventing cervical cancer by changing vaginal microflora.Methods Patients infected with a single subtype of high-risk human papillomavirus(HR-HPV)a9 were selected from the colposcopy clinic of Beijing Chaoyang Hospital Affiliated to Capital Medical University.A total of 207 patients with low-grade squamous intraepithelial lesion or normal(LSIL-group)and 117 patients with high-grade squamous intraepithelial lesion or invasive carcinoma(HSIL+group)were enrolled in this study.Vaginal microbiota characteristics were compared between the two groups of subjects by application of 16 s rRNA gene sequencing V4 area technical.Rank sum test,chi-square test,curve fitting and Linear discriminant analysis Effect Size(LEfSe)were used to analyze the relationship between vaginal microbiota characteristics and different degree of cervical lesions in women infected with HPV-a9.Results There was statisticallysignificant difference in HPV subtypes between HSIL+group and LSIL-group(P＜0.05).There was no significant difference in the number of species and Shannon index of vaginal microbiota between the two groups(P＞0.05).There are different trends in the distribution of vaginal flora in different lesions.The relative abundance of Prevotella in LSIL-group was higher than that in HSIL+group,and the difference was statistically significant(Z=2.59,P＜0.05).LEfSe analysis showed that Staphylococcus was significantly higher in the HSIL+group than in the LSIL-group,and Prevotella was significantly higher in the LSIL-group than in the HSIL+group.Stratified analysis showed that the risk of high-grade lesions with different relative abundance of Prevotella was not equal when the age of first sexual intercourse less than 20 years old,the number of births less than 3,no family history of malignant tumors,the education level higher than undergraduate,and the BMI less than 24 kg/m2(P＜0.05).Conclusions The composition of vaginal microbiota is different in patients with different degrees of cervical lesions,and there may be internal balance among multiple bacterial genera.The abundance of Staphylococcus is high in severe cervical lesions.The increased abundance of Prevotella is closely related to normal cervix and low-grade lesions,and is affected by many factors.
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