Objective To investigate the effect and mechanism of menstrual blood-derived mesenchymal stem cells(MenSCs)derived exosomes(MenSCs-Exos)on proliferation,migration,invasion and cell cycle distribution of human ovarian cancer cell line A2780.Methods MenSCs were isolated and purified,cell phenotype was identified by flow cytometry,adipogenic differentiation ability was observed by red oil O staining,MenSCs-Exos were isolated by ultracentrifugation,the morphology of exosomes were observed by electron microscope,the size and distribution were analyzed by NTA,and the expression of CD9,calnexin and TSG101 were detected by Western blot.The effects of MenSCs-Exos on the proliferation,migration,invasion and cell cycle distribution of A2780 cells were detected by CCK-8 assay,wound healing assay,Transwell migration and invasion assay and flow cytometry.The expression of Wnt/β-catenin signaling pathway related proteins and migration related proteins were detected by Western blot.Results MenSCs with stem cell phenotype,osteogenic and adipogenic differentiation potential were successfully isolated.At the same time,MenSCs-Exos was found a membranous vesicle with clear edge and uniform size distribution,the diameter of which was about 30-150 nm,with an average of 72.89 nm.The expression of CD9 and TSG101 on the membrane surface was positive,while the expression of calnexin was negative.Compared with the control group,MenSCs-Exos treatment group could enhance the ability of proliferation,migration and invasion of A2780 cells,affect the distribution of cell cycle and up-regulate the expression of β-catenin,c-Myc,TCF4,CyclinD1,Vimentin and N-cadherin,and down-regulate the expression of E-cadherin.Conclusion MenSCs-Exos may promote the proliferation of ovarian cancer cells by activating Wnt/β-catenin signal pathway and promote the migration and invasion of A2780 cells by up-regulating the expression of E-cadherin.
关键词
外泌体/经血源间充质干细胞/卵巢癌/Wnt/β-catenin信号通路
Key words
Exosomes/Menstrual blood-derived mesenchymal stem cells/Ovarian cancer/Wnt/β-catenin signal pathway
维普
万方数据