Objective To explore the biocompatibility of magnetotactic bacteria AMB-1 in BALB/c mice under in vitro and in vivo conditions.Methods The morphological characteristics of AMB-1 were observed in vitro using transmission electron microscopy(TEM).The morphology of AMB-1 was observed by transmission electron microscopy.The motion of AMB-1 in serum was captured by microscope,and the motion speed was calculated by Image J.The cytotoxicity of AMB-1 to mouse breast cancer cells 4T1 and mouse fibroblast cells L929 was assessed using the LDH assay.The effect of AMB-1 on BALB/c mouse bone marrow-derived dendritic cells(BMDC)was evaluated using flow cytometry.The median lethal dose(LD50)of AMB-1 in BALB/c mouse was calculated using the Karber's method.The distribution of AMB-1 in BALB/c mouse organs(heart,liver,spleen,lung,and kidney)was observed by Prussian Blue staining.The effect of AMB-1 on splenic dendritic cells(DC)in the BALB/c mouse spleen was detected by flow cytometry,the safety of AMB-1 was assessed by testing the biochemical indices in mouse serum.And the histological changes in the major organs of the mice were observed using Hematoxylin and Eosin staining.Results Under 37℃ conditions,AMB-1 could maintain its movement in serum for at least 120 minutes.When the concentration of AMB-1 exceeded 3.3×106,it was toxic to mouse mammary cancer cells 4T1 and fibroblasts L929,and the toxicity increased with the increase of bacteria amount.AMB-1 could promote the maturation of bone marrow-derived dendritic cells.The LD50 of AMB-1 for BALB/c mice was 2.59×1010.The effect of AMB-1 on the maturation of dendritic cells in the mouse spleen was gradually weakened with time.Blood biochemical indicators were within normal ranges.There were no significant histomorphological changes in the major organs of the mice.Conclusions Magnetotactic bacteria AMB-1 possess relatively good biocompatibility.They do not induce severe immune inflammation or other adverse reactions in vivo,demonstrating promising potential for in vivo applications.
维普
万方数据