水溶性蛋白盐析的一维结晶
One-dimensional Crystallization of Salting Out Water-soluble Protein
杨光弟 1周红锋 1靳林2
作者信息
- 1. 临沧师范高等专科学校,云南,临沧,677000
- 2. 临沧师范高等专科学校,云南,临沧,677000;中山大学工学院,广东,广州,510006
- 折叠
摘要
目的:研究水溶性蛋白质盐析结晶的形态,揭示水溶性蛋白盐析一维结晶的物理机理.方法:选择白蛋白标准液,人血清,人血红蛋白,乙肝丙种球蛋白,卵清蛋白等水溶性蛋白质进行二维盐析,用显微摄影技术得到显微摄影图片,再对结晶的显微图像进行分析.结果:水溶性蛋白质盐析结晶的形态一般情况下具有无标度区间,具有屏蔽效应,具有典型的分形特征;但是,在蛋白质浓度较低,并直接滴在载玻片时,也观察到非分形结构的一维结晶.结论:水溶性蛋白涂片盐析结晶显微图片的分析发现,水溶性蛋白涂片盐析结晶一般形成分形结晶,但在特定情况下可形成一维结晶,通过对水溶性蛋白盐析的一维结晶进行理论分析,用电偶极子模型从理论上解释了蛋白质一维结晶形成机理.
Abstract
Objective: The habit of water-soluble protein salting out crystallizes was researched and the physical mechanism of one-dimensional crystallization of salting out water-soluble protein is revealed. Methods: The albumin standard solution, human serum, human hemoglobin, hepatitis B gamma globulin, ovalbumin and other water-soluble protein were analyzed by means of two-dimensional salting. And Pictures of these. Protein were obtained by microscopic photography, then Microscopic Pictures of crystal were analyze. Results: Water-soluble form of protein crystallization of salt generally has no marked the interval, shielding effect, typical fractal characteristic; however when protein eoncentration is lower and directly onto the glass slide,the presumptuous shaped structure of one-dimensional crystals can be observed. Conclusions: Based on analysis water-soluble protein of the digitalized image of scanning electron microscope (SEM) micrographs, the result shows: water-soluble protein fraetal geometry crystallization, however, one-dimensional crystallization can produce at the special condition. The theoretical analysis of water-soluble salt by one-dimensional protein crystals is made, one-dimensional protein crystallization mechanism is explained with electric dipole model.
关键词
分形/水溶性蛋白/盐析/电偶极子模型/一维结晶/相互作用能Key words
fractal geometry/water-soluble protein/salting out/electric dipole model/one-dimensional crystal/interaction energy引用本文复制引用
基金项目
云南省教育厅自然科学基金资助项目(2010Y210)
出版年
2011
NETL
NSTL
维普
万方数据