Ca2+在电磁场调控成骨细胞增殖与分化中的作用
Role of Ca2+in electromagnetic field regulation on osteoblast proliferation and differentiation
张广伟 1梁卓文 2杨治3
作者信息
- 1. 陕西中医药大学第二临床医学院,陕西咸阳 712046
- 2. 空军军医大学西京医院骨科,陕西西安 710032
- 3. 红会医院关节病医院关节翻修病区,陕西西安 710054
- 折叠
摘要
目的:从定性和定量的角度探究电磁场(EMF)对成骨细胞内Ca2+变化的影响,并试图探明Ca2+在电磁场调控成骨细胞增殖与分化中的作用.方法:搭建频率为38.7 Hz,强度为1.5 mT的正弦EMF发生平台,将MC3T3-E1成骨细胞随机分为对照组和实验(EMF)组.EMF组施加EMF干预,每天干预8h.利用CCK8检测成骨细胞增殖,ALP染色检测成骨细胞分化,Ca2+荧光探针和流式细胞仪检测成骨细胞内Ca2+浓度.结果:CCK8细胞增殖结果显示,EMF干预48、72、96及120 h,可显著促进成骨细胞增殖.ALP染色结果显示EMF干预14d后,EMF组ALP表达显著高于对照组.Ca2+荧光探针及流式细胞仪检测Ca2+浓度结果显示EMF干预可促进成骨细胞内Ca2+的增多.结论:EMF上调成骨细胞内Ca2+信号可能与EMF促成骨细胞增殖和分化密切相关,但是哪些与Ca2+相关的生物信号通路参与了EMF促成骨细胞增殖和分化效应,有待进一步研究.
Abstract
Objective To explore the effects of electromagnetic field(EMF)on thechange of Ca2+ in osteolbast from the qualitative and quantitative perspectives,and try to identify the role of Ca2+in EMF regulation on osteoblast proliferation and differentiation.Methods A platform was established for generating sine EMF with a frequency of 38.7 Hz and a strength of 1.5 mT.The MC3T3-E1 osteoblasts were randomly divided into control group and experimental group(EMF intervention for 8 h per day).CCK8 was used to detect osteoblast proliferation,ALP staining to detect osteoblast differentiation,and Ca2+fluorescence probes and flow cytometer to detect the Ca2+concentration in osteoblasts.Results CCK8 result showed that EMF intervention for 48,72,96 and 120 h could significantly promote osteoblast proliferation.After 14 days of EMF intervention,the positive expression of ALP was significantly higher in EMF group than in control group.Ca2+fluorescent staining and flow cytometry results revealed that EMF intervention could increase the Ca2+in osteoblasts.Conclusion The EMF-induced upregulation of Ca2+ signal in osteoblasts may be closely related to the promotions of osteoblast proliferation and differentiation by EMF,but which Ca2+-related biosignaling pathways are involved in the EMF promoting osteoblast proliferation and differentiation remains to be further investigated.
关键词
电磁场/成骨细胞/钙离子/细胞增值/细胞分化Key words
electromagnetic field/osteoblast/calcium ion/cell proliferation/cell differentiation引用本文复制引用
出版年
2024
NETL
NSTL
万方数据