基于LQ模型的原发性肝癌立体定向放射治疗剂量学实验
Experimental study of stereotactic body radiotherapy dosimetry for primary hepatic carcinoma based on LQ model
万福莺 1周凌宏2
作者信息
- 1. 南方医科大学生物医学工程学院,广东广州 510515;同济大学附属妇产科医院放射治疗科,上海 200092
- 2. 南方医科大学生物医学工程学院,广东广州 510515
- 折叠
摘要
目的:探讨肝癌立体定向放射治疗(SBRT)的剂量学效应是否符合线性二次(LQ)模型.方法:选取体外培养的人肝癌细胞株HepG2和Hep3B,给予生物有效剂量(BED)照射(6、8、10、12、14 Gy).照射分割方式包括单次照射(模拟SBRT)和分次照射3次、5次(模拟IMRT).检测辐照后存活分数反应不同分割方式的损伤效应;绘制生存曲线,观察不同分割方式对细胞增殖的影响;辐照后细胞划痕实验,观察不同分割方式对细胞侵袭和迁移能力的影响.结果:LQ模型在肝癌SBRT放疗的应用体外验证中出现明显的分离效应.对于HepG2细胞和Hep3B细胞,当BED<12 Gy(α/β=10 Gy)时,不同分割方式在损伤效应、增殖能力、侵袭能力上未显示出明显差异,SBRT符合LQ模型;当BED≥12 Gy时,单次分割相对于多次分割,显示出更高的损伤效应,即SBRT损伤效应高于IMRT.结论:SBRT在肝癌中适用LQ模型具有一定的剂量区间,超过该区间损伤效应高于LQ模型预测结果.
Abstract
Objective To investigate whether the dosimetric effects of stereotactic body radiotherapy(SBRT)in hepatic carcinoma conform to the linear-quadratic(LQ)model.Methods Human hepatic carcinoma cell lines HepG2 and Hep3B cultured in vitro were selected and subjected to biological equivalent dose(BED)irradiation(6,8,10,12,14 Gy).The fractionation regimens included single-fraction irradiation(simulated SBRT)and 3-or 5-fraction irradiation(simulated IMRT).The surviving fraction after irradiation reflected the damage effects of different fractionation regimens;the effects of different fractionation regimens on cell proliferation were analyzed through survival curves;and cell scratch experiment after irradiation was used to observe the cell invasive and migration abilities after exposure to different fractionation regimens.Results Significant separation effect was observed in the in vitro validation of the LQ model in SBRT for hepatic carcinoma.For HepG2 cells and Hep3B cells,when BED<12 Gy(α/β=10 Gy),fractionation regimens did not show significant differences in terms of damage effects,proliferative ability,and invasive ability,indicating SBRT conformed to the LQ model;when BED≥12 Gy,single-fraction had more obvious damage effects as compared with multiple-fraction,indicating that the damage effect in SBRT was more significant than that in IMRT.Conclusion The LQ model applies to SBRT for hepatic carcinoma in a certain dose range,beyond which the damage effect is higher than the predicted results of the LQ model.
关键词
肝癌/线性二次模型/剂量分割/立体定向放射治疗Key words
hepatic carcinoma/linear-quadratic model/fractionation regimen/stereotactic body radiotherapy引用本文复制引用
出版年
2024
NETL
NSTL
万方数据